Supplementary Figures S1-S5 and Supplementary Methods from Systematic siRNA Screen Unmasks NSCLC Growth Dependence by Palmitoyltransferase DHHC5

Supplementary Figures S1-S5 and Supplementary Methods. Figure S1: Screening of a large number of NSCLC lines for sensitivity to siRNAs directed against 23 DHHC palmitoyltransferases. Figure S2: Membrane localization of DHHC5 in DHHC5 and DHHS mutant overexpressing cells. Figure S3: Anchorage-independent colony formation (soft agar). Figure S4: DHHC5 immunoblotting (A) and immunohistochemistry (B) of H1299 xenografts developing from H1299 control (CON) or H1299 DHHC5 knockdown cells rescued with a DHHC5-expressing plasmid. Figure S5: DHHC5 immunoblotting (A) and immunohistochemistry (B) of H358 xenografts developing from control (CON) or DHHC5 knockdown (KD) cells.