Back to Search
Start Over
The expression and function of opsin genes related to the phototactic behavior of Asian citrus psyllid
- Source :
- Pest Management Science. 76:1578-1587
- Publication Year :
- 2019
- Publisher :
- Wiley, 2019.
-
Abstract
- Background The Asian citrus psyllid (ACP), Diaphorina citri Kuwayama (Hemiptera: Psyllidae), is a prominent pest of citrus because it transmits Candidatus Liberibacter asiaticus associated with huanglongbing. Phototactic behavior ubiquitously exists in insects and plays essential roles in host-searching and mate-searching in ACP. However, its mechanism has remained unclear to date. In this paper, molecular assays were performed to understand the relation between opsins and the phototactic behavior of ACP. Results We cloned five opsin genes and analyzed their expression patterns. The main three opsin genes (Dc-UV, Dc-BW and Dc-LW) displayed high expression levels in daytime, and the expression levels of the other two opsin genes (Dc-UV-like and Dc-arthropsin) increased during nighttime. In constant darkness, the expression patterns and levels of opsin genes of ACP were altered. Under starvation, the expression levels of the main three opsins were reduced (53.89-84.10%). In contrast, the expression levels in females increased (27.55-60.58%). Feeding of double-stranded RNA (Dc-UV, Dc-LW or Dc-BW) have reduced the phototactic efficiency of ACP to ultraviolet light (44.79%), green light (54.81%) and blue light (43.00%), respectively. Conclusion The results suggest that the expression levels and patterns of opsins of ACP were influenced by photoperiod and the physiological status of ACP. Our research indicates that opsins play crucial roles in phototactic behavior in ACP. © 2019 Society of Chemical Industry.
- Subjects :
- 0106 biological sciences
Citrus
Opsin
Diaphorina citri
01 natural sciences
Hemiptera
Rhizobiaceae
RNA interference
Ultraviolet light
Phototaxis
Animals
Gene
Plant Diseases
RNA, Double-Stranded
Genetics
photoperiodism
Opsins
biology
General Medicine
biology.organism_classification
010602 entomology
Insect Science
Female
PEST analysis
Agronomy and Crop Science
010606 plant biology & botany
Subjects
Details
- ISSN :
- 15264998 and 1526498X
- Volume :
- 76
- Database :
- OpenAIRE
- Journal :
- Pest Management Science
- Accession number :
- edsair.doi.dedup.....059505eed69f0704aa4bdcd14734c8fd