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Automated and manual patch clamp data of human induced pluripotent stem cell-derived dopaminergic neurons
- Source :
- Scientific Data
- Publication Year :
- 2017
-
Abstract
- Human induced pluripotent stem cells can be differentiated into dopaminergic neurons (Dopa.4U). Dopa.4U neurons expressed voltage-gated NaV and KV channels and showed neuron-like spontaneous electrical activity. In automated patch clamp measurements with suspended Dopa.4U neurons, delayed rectifier K+ current (delayed KV) and rapidly inactivating A-type K+ current (fast KV) were identified. Examination of the fast KV current with inhibitors yielded IC50 values of 0.4 mM (4-aminopyridine) and 0.1 mM (tetraethylammonium). In manual patch clamp measurements with adherent Dopa.4U neurons, fast KV current could not be detected, while the delayed KV current showed an IC50 of 2 mM for 4-aminopyridine. The NaV channels in adherent and suspended Dopa.4U neurons showed IC50 values for tetrodotoxin of 27 and 2.9 nM, respectively. GABA-induced currents that could be observed in adherent Dopa.4U neurons could not be detected in suspended cells. Application of current pulses induced action potentials in approx. 70 % of the cells. Our results proved the feasibility of automated electrophysiological characterization of neuronal cells.
- Subjects :
- 0301 basic medicine
Statistics and Probability
Data Descriptor
Patch-Clamp Techniques
Potassium Channels
Cellular differentiation
Library and Information Sciences
Ion channels in the nervous system
Ion Channels
Education
03 medical and health sciences
chemistry.chemical_compound
0302 clinical medicine
Automated patch clamp
Humans
Patch clamp
Induced pluripotent stem cell
Ion channel
Ion transport
Tetraethylammonium
Chemistry
Dopaminergic Neurons
Cell Differentiation
Anatomy
Computer Science Applications
Electrophysiology
Induced pluripotent stem cells
030104 developmental biology
Biophysics
Tetrodotoxin
Statistics, Probability and Uncertainty
030217 neurology & neurosurgery
Information Systems
Subjects
Details
- ISSN :
- 20524463
- Volume :
- 4
- Database :
- OpenAIRE
- Journal :
- Scientific data
- Accession number :
- edsair.doi.dedup.....05921c116c3cce654f2d410a76e5ccfd