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Purification, crystallization and preliminary X-ray diffraction analysis of Imp3 in complex with an Mpp10 peptide involved in yeast ribosome biogenesis

Authors :
Keqiong Ye
Sanduo Zheng
Source :
Acta crystallographica. Section F, Structural biology communications. 70(Pt 7)
Publication Year :
2014

Abstract

Eukaryotic ribosome synthesis requires a vast number of transiently associated factors. Mpp10, Imp3 and Imp4 form a protein complex in the 90S pre-ribosomal particle that conducts early processing of 18S rRNA. Here, a short fragment of Mpp10 was identified to associate with and increase the solubility of Imp3. An Imp3–Mpp10 complex was co-expressed, co-purified and co-crystallized. Preliminary X-ray diffraction analysis revealed that the crystal diffracted to 2.1 Å resolution and belonged to space groupP212121, with unit-cell parametersa= 51.6,b= 86.9,c= 88.7 Å.

Details

ISSN :
2053230X
Volume :
70
Issue :
Pt 7
Database :
OpenAIRE
Journal :
Acta crystallographica. Section F, Structural biology communications
Accession number :
edsair.doi.dedup.....057f54b42f8de472651888fe8a1b3a74