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Variation of 4 MV X-ray dose rate in fractionated irradiation strongly impacts biological endothelial cell response
- Source :
- International journal of radiation biology. 98(1)
- Publication Year :
- 2021
-
Abstract
- Purpose Even though X-ray beams are widely used in medical diagnosis or radiotherapy, the comparisons of their dose rates are scarce. We have recently demonstrated in vitro (clonogenic assay, cell viability, cell cycle, senescence) and in vivo (weight follow-up of animals and bordering epithelium staining of lesion), that for a single dose of irradiation, the relative biological effectiveness (RBE) deviates from 1 (up to twofold greater severe damage at the highest dose rate depending on the assay) when increasing the dose rate of high energy X-ray beams. Material and methods To further investigate the impact of the dose rate on RBE, in this study we performed in vitro fractionated irradiations by using the same two dose rates (0.63 and 2.5 Gy.min-1) of high-energy X-rays (both at 4 MV) on normal endothelial cells (HUVECs). We investigated the viability/mortality, characterized radiation-induced senescence by using flow cytometry and measured gene analysis deregulations on custom arrays. Results The overall results enlighten that, in fractionated irradiations when varying the dose rate of high-energy X-rays, the RBE of photons deviates from 1 (up to 2.86 for viability/mortality experiments performed 21 days post-irradiation). Conclusion These results strengthen the interest of multiparametric analysis approaches in providing an accurate evaluation of the outcomes of irradiated cells in support of clonogenic assays, especially when such assays are not feasible.
- Subjects :
- Radiological and Ultrasound Technology
medicine.diagnostic_test
Chemistry
Cell Survival
X-Rays
Endothelial Cells
Dose-Response Relationship, Radiation
Cell cycle
In vitro
Flow cytometry
Andrology
Endothelial stem cell
In vivo
medicine
Relative biological effectiveness
Animals
Radiology, Nuclear Medicine and imaging
Viability assay
Clonogenic assay
Relative Biological Effectiveness
Subjects
Details
- ISSN :
- 13623095
- Volume :
- 98
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- International journal of radiation biology
- Accession number :
- edsair.doi.dedup.....054e19fa40f386f03a28be676fc31366