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TRIM5alpha association with cytoplasmic bodies is not required for antiretroviral activity
- Source :
- Virology. 343(2)
- Publication Year :
- 2005
-
Abstract
- The tripartite motif (TRIM) protein, TRIM5alpha, restricts infection by particular retroviruses. Many TRIM proteins form cytoplasmic bodies of unknown function. We investigated the relationship between cytoplasmic body formation and the structure and antiretroviral activity of TRIM5alpha. In addition to diffuse cytoplasmic staining, the TRIM5alpha proteins from several primate species were located in cytoplasmic bodies of different sizes; by contrast, TRIM5alpha from spider monkeys did not form cytoplasmic bodies. Despite these differences, all of the TRIM5alpha proteins exhibited the ability to restrict infection by particular retroviruses. Treatment of cells with geldanamycin, an Hsp90 inhibitor, resulted in disappearance or reduction of the TRIM5alpha-associated cytoplasmic bodies, yet exerted little effect on the restriction of retroviral infection. Studies of green fluorescent protein-TRIM5alpha fusion proteins indicated that no TRIM5alpha domain is specifically required for association with cytoplasmic bodies. Apparently, the formation of cytoplasmic bodies is not required for the antiretroviral activity of TRIM5alpha.
- Subjects :
- Geldanamycin
Cytoplasm
Cytoplasmic bodies
viruses
Lactams, Macrocyclic
Recombinant Fusion Proteins
Ubiquitin-Protein Ligases
Cytoplasmic speckles
DNA, Recombinant
Biology
Hsp90 inhibitor
Antiviral Restriction Factors
Tripartite Motif Proteins
chemistry.chemical_compound
Retrovirus
TRIM5α
Species Specificity
Virology
Chlorocebus aethiops
Restriction
Benzoquinones
Animals
Humans
HSP90 Heat-Shock Proteins
Saimiri
Base Sequence
Quinones
Proteins
Tripartite motif
biology.organism_classification
Fusion protein
Tripartite motif family
Molecular biology
Macaca mulatta
Microscopy, Electron
chemistry
Anti-Retroviral Agents
Microscopy, Fluorescence
Cebidae
HIV-1
biology.protein
TRIM5alpha
Carrier Proteins
HeLa Cells
Subjects
Details
- ISSN :
- 00426822
- Volume :
- 343
- Issue :
- 2
- Database :
- OpenAIRE
- Journal :
- Virology
- Accession number :
- edsair.doi.dedup.....054155b2f26acb8c78ecf14e95961073