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NAT, a Human Complex Containing Srb Polypeptides that Functions as a Negative Regulator of Activated Transcription
- Source :
- Molecular Cell. 2:213-222
- Publication Year :
- 1998
- Publisher :
- Elsevier BV, 1998.
-
Abstract
- A complex that represses activated transcription and contains the human homologs of the yeast Srb7, Srb10, Srb11, Rgr1, and Med6 proteins was isolated. The complex is devoid of the Srb polypeptides previously shown to be components of the yeast Mediator complex that functions in transcriptional activation. The complex phosphorylates the CTD of RNA polymerase II (RNAPII) at residues other than those phosphorylated by the kinase of TFIIH. Moreover, the complex specifically interacts with RNAPII. The interaction is not mediated by the CTD of RNAPII, but is precluded by phosphorylation of the CTD. Our results indicate that the complex is a subcomplex of the human RNAPII holoenzyme. We suggest that the RNAPII holoenzyme is a transcriptional control panel, integrating and responding to specific signals to activate or repress transcription.
- Subjects :
- Transcriptional Activation
DNA, Complementary
Saccharomyces cerevisiae Proteins
Macromolecular Substances
Molecular Sequence Data
Mediator Complex Subunit 1
RNA polymerase II
MED6
Biology
environment and public health
MED1
Fungal Proteins
Mediator
Transcription (biology)
Cyclins
Transcriptional regulation
Animals
Humans
Amino Acid Sequence
Phosphorylation
Molecular Biology
Mediator Complex
Base Sequence
Sequence Homology, Amino Acid
Cell Biology
Molecular biology
Cyclin-Dependent Kinases
Cell biology
Repressor Proteins
enzymes and coenzymes (carbohydrates)
Trans-Activators
Transcription factor II H
biology.protein
RNA Polymerase II
Transcription Factors
Subjects
Details
- ISSN :
- 10972765
- Volume :
- 2
- Database :
- OpenAIRE
- Journal :
- Molecular Cell
- Accession number :
- edsair.doi.dedup.....051d7c1945f463f2a05f73b534bc058e