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Monitoring creatine and phosphocreatine by 13C MR spectroscopic imaging during and after 13C4 creatine loading: a feasibility study
- Source :
- Amino Acids, Amino Acids, 48, 1857-66, Amino Acids, 48, 8, pp. 1857-66
- Publication Year :
- 2016
- Publisher :
- Springer Vienna, 2016.
-
Abstract
- Contains fulltext : 167953.pdf (Publisher’s version ) (Open Access) Creatine (Cr) supplementation to enhance muscle performance shows variable responses among individuals and different muscles. Direct monitoring of the supplied Cr in muscles would address these differences. In this feasibility study, we introduce in vivo 3D (13)C MR spectroscopic imaging (MRSI) of the leg with oral ingestion of (13)C4-creatine to observe simultaneously Cr and phosphocreatine (PCr) for assessing Cr uptake, turnover, and the ratio PCr over total Cr (TCr) in individual muscles. (13)C MRSI was performed of five muscles in the posterior thigh in seven subjects (two males and two females of ~20 years, one 82-year-old male, and two neuromuscular patients) with a (1)H/(13)C coil in a 3T MR system before, during and after intake of 15 % (13)C4-enriched Cr. Subjects ingested 20 g Cr/day for 4 days in four 5 g doses at equal time intervals. The PCr/TCr did not vary significantly during supplementation and was similar for all subjects and investigated muscles (average 0.71 +/- 0.07), except for the adductor magnus (0.64 +/- 0.03). The average Cr turnover rate, assessed in male muscles, was 2.1 +/- 0.7 %/day. The linear uptake rates of Cr were variable between muscles, although not significantly different. This assessment was possible in all investigated muscles of young male volunteers, but less so in muscles of the other subjects due to lower signal-to-noise ratio. Improvements for future studies are discussed. In vivo (13)C MRSI after (13)C-Cr ingestion is demonstrated for longitudinal studies of Cr uptake, turnover, and PCr/TCr ratios of individual muscles in one exam.
- Subjects :
- 0301 basic medicine
In vivo magnetic resonance spectroscopy
Adult
Male
medicine.medical_specialty
Pathology
Magnetic Resonance Spectroscopy
Phosphocreatine
Clinical Biochemistry
Creatine
Biochemistry
03 medical and health sciences
chemistry.chemical_compound
0302 clinical medicine
In vivo
Internal medicine
Urological cancers Radboud Institute for Molecular Life Sciences [Radboudumc 15]
medicine
Ingestion
Humans
13C
Human muscle
Muscle, Skeletal
Aged, 80 and over
Carbon Isotopes
business.industry
Other Research Radboud Institute for Health Sciences [Radboudumc 0]
Metabolic Disorders Radboud Institute for Health Sciences [Radboudumc 6]
Organic Chemistry
MR spectroscopy
Posterior compartment of thigh
Disorders of movement Donders Center for Medical Neuroscience [Radboudumc 3]
030104 developmental biology
Endocrinology
Oral ingestion
chemistry
Mr spectroscopic imaging
Original Article
Female
business
030217 neurology & neurosurgery
Subjects
Details
- Language :
- English
- ISSN :
- 14382199 and 09394451
- Volume :
- 48
- Database :
- OpenAIRE
- Journal :
- Amino Acids
- Accession number :
- edsair.doi.dedup.....051c6786e9db055958adb00d7b9cfa23