Interference in glucose oxidase-peroxidase blood glucose methods

Interference by certain compounds with the glucose oxidase-peroxidase system used in blood glucose estimations appears to be a function of the oxygen affinity of the oxygen acceptor chromogen or the oxidative coupling system. It has been shown 1 that tolazamide interferes seriously with the Boehringer GOD-PERID blood glucose method. By utilising other compounds with similar chemical groups, it was found possibleto identify the group in the tolazamide molecule that caused interference and consequently to predict the effects of other compounds on such blood glucose methods. A comparison of three chromogenic systems was also performed. These were guaiacum 2 , phenol-4-aminophenazone 3 and the Boehringer 2'2-diazo-di(3-ethyl benzthiazoline-6-sulphonic acid) (ABTS). Some of the advantages and disadvantages of these three systems are discussed.