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Molecular characterization of environmental Cryptococcus neoformans VNII isolates in Jos, Plateau State, Nigeria
- Publication Year :
- 2016
-
Abstract
- Cryptococcus neoformans and Cryptococcus gattii are encapsulated yeasts able to cause fatal neurological infections in both human and other mammals. Cryptococcosis is the most common fungal infection of the central nervous system and has a huge burden in sub-Saharan Africa and South East Asia. Bird excreta are considered an environmental reservoir for C. neoformans in urban areas, therefore a study aimed at isolating and characterizing this yeast is important in disease management. In this study, one hundred samples of pigeon droppings were collected in Jos, Plateau State, Nigeria. C. neoformans was isolated from three samples and initially identified using standard phenotypic and biochemical tests. Molecular analysis revealed that all three isolates belonged to C. neoformans genotype VNII, mating type α and were assigned to the sequence type ST43 by multilocus sequence typing analysis. This study reports, for the first time, the molecular characterization of C. neoformans in Nigeria, where little is still known about the environmental distribution of the genotypes, serotypes and mating types of this important human pathogen.
- Subjects :
- 0301 basic medicine
Serotype
Mating type
Antifungal Agents
030106 microbiology
Nigeria
Human pathogen
Microbial Sensitivity Tests
Cryptococcus neoformans
Environment
Mating types
Molecular types
Infectious Diseases
Microbiology
Feces
03 medical and health sciences
parasitic diseases
Genotype
Environmental Microbiology
medicine
Animals
Humans
Columbidae
DNA, Fungal
Mycological Typing Techniques
Cryptococcus gattii
biology
Cryptococcosis
Genes, Mating Type, Fungal
biology.organism_classification
medicine.disease
Molecular Typing
Multilocus sequence typing
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Accession number :
- edsair.doi.dedup.....05093af12fb2ba8a72dcc3264f9075e0