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Endothelial semaphorin 3F maintains endothelial barrier function and inhibits monocyte migration
- Source :
- International Journal of Molecular Sciences; Volume 21; Issue 4; Pages: 1471, International Journal of Molecular Sciences, 21(4). MDPI, International Journal of Molecular Sciences, Vol 21, Iss 4, p 1471 (2020), International Journal of Molecular Sciences
- Publication Year :
- 2020
-
Abstract
- In normal physiology, endothelial cells (ECs) form a vital barrier between the blood and underlying tissue controlling leukocyte diapedesis and vascular inflammation. Emerging data suggest that neuronal guidance cues, typically expressed during development, have roles outside the nervous system in vascular biology and immune responses. In particular, Class III semaphorins have been reported to affect EC migration and angiogenesis. While ECs express high levels of semaphorin 3F (SEMA3F), little is known about its function in mature ECs. Here we show that SEMA3F expression is reduced by inflammatory stimuli and increased by laminar flow. Endothelial cells exposed to laminar flow secrete SEMA3F, which subsequently binds to heparan sulfates on the surface of ECs. However, under pro-inflammatory conditions, reduced levels of SEMA3F make ECs more prone to monocyte diapedesis and display impaired barrier function as measured with an electric cell–substrate impedance sensing system and a microfluidic system. In addition, we demonstrate that SEMA3F can directly inhibit the migration of activated monocytes. Taken together, our data suggest an important homeostatic function for EC-expressed SEMA3F, serving as a mediator of endothelial quiescence.
- Subjects :
- Nervous system
Angiogenesis
Nerve Tissue Proteins
semaphorin
Article
Catalysis
lcsh:Chemistry
Inorganic Chemistry
Mediator
Immune system
Semaphorin
Human Umbilical Vein Endothelial Cells
medicine
Humans
Physical and Theoretical Chemistry
lcsh:QH301-705.5
Molecular Biology
Spectroscopy
Barrier function
Inflammation
Chemistry
Monocyte
Organic Chemistry
endothelial cells
monocytes
Transendothelial and Transepithelial Migration
Membrane Proteins
General Medicine
Computer Science Applications
Cell biology
HEK293 Cells
medicine.anatomical_structure
lcsh:Biology (General)
lcsh:QD1-999
Endothelium, Vascular
Homeostasis
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Journal :
- International Journal of Molecular Sciences; Volume 21; Issue 4; Pages: 1471, International Journal of Molecular Sciences, 21(4). MDPI, International Journal of Molecular Sciences, Vol 21, Iss 4, p 1471 (2020), International Journal of Molecular Sciences
- Accession number :
- edsair.doi.dedup.....05053cea0c7da65552fdb1f92e73f570