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Identification of glycoproteins on nitrocellulose membranes and gels
- Source :
- Methods in molecular biology (Clifton, N.J.). 32
- Publication Year :
- 1994
-
Abstract
- In this article we describe a procedure for the detection of glycoproteins on gels employing the periodic acid-Schiff's reagent. In addition, a number of staining protocols and direct binding ELISA, employing antibodies and lectins, are described for the identification and quantitation of glycoproteins after their immobilization by dot, slot, or Western blotting onto nitrocellulose membranes. We document, in detail, the conditions (i.e., the effect of solvent and detergents) for the immobilization of one specific family of O-linked glycoproteins, namely mucins. However, taking into account our suggestions, these procedures should be applicable to other types of glycoprotein.
- Subjects :
- Blotting, Western
Acrylic Resins
Enzyme-Linked Immunosorbent Assay
Bioengineering
Applied Microbiology and Biotechnology
Biochemistry
Sepharose
chemistry.chemical_compound
Collodion
Lectins
Periodic Acid-Schiff Reaction
Molecular Biology
Polyacrylamide gel electrophoresis
Glycoproteins
chemistry.chemical_classification
Electrophoresis, Agar Gel
Chromatography
Staining and Labeling
Chemistry
Mucin
Mucins
Membranes, Artificial
Membrane
Electrophoresis, Polyacrylamide Gel
Alcian Blue
Glycoprotein
Gels
Nitrocellulose
Biotechnology
Subjects
Details
- ISSN :
- 10643745
- Volume :
- 32
- Database :
- OpenAIRE
- Journal :
- Methods in molecular biology (Clifton, N.J.)
- Accession number :
- edsair.doi.dedup.....04f7545ba7f199a692df67f90b20a3bc