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Venomic analysis and evaluation of antivenom cross-reactivity of South American Micrurus species

Venomic analysis and evaluation of antivenom cross-reactivity of South American Micrurus species

Authors :
Giselle Agostini Cotta
Breno Rates
Daniel A. F. Silva
Paula Henriques Cruz Ciscotto
Maria Elena de Lima
Luciano P. Silva
Eladio F. Sanchez
Micheline Freire Donato
Hélida Monteiro de Andrade
Raquel J. Rodrigues
Wany Selena Maria
Michael K. Richardson
Adriano Monteiro de Castro Pimenta
Source :
Journal of proteomics. 74(9)
Publication Year :
2011

Abstract

Coral snakes from Micrurus genus are the main representatives of the Elapidae family in South America. However, biochemical and pharmacological features regarding their venom constituents remain poorly investigated. Here, venomic analyses were carried out aiming at a deeper understanding on the composition of M. frontalis , M. ibiboboca , and M. lemniscatus venoms. In the three venoms investigated, proteins ranging from 6 to 8 kDa (3FTx) and 12 to 14 kDa (PLA 2 ) were found to be the most abundant. Also, the N-terminal sequences of four new proteins, purified from the M. lemniscatus venom, similar to 3FTx, PLA 2 and Kunitz-type protease inhibitor from other Micrurus and elapid venoms are reported. Cross-reactivity among different Micrurus venoms and homologous or heterologous antivenoms was carried out by means of 2D-electrophoresis and immunoblotting. As, expected, the heterologous anti-Elapid venom displayed the highest degree of cross-reactivity. Conversely, anti- M. corallinus reacted weakly against the tested venoms. In gel digestions, followed by mass spectrometry sequencing and similarity searching, revealed the most immunogenic protein families as similar to short and long neurotoxins, weak neurotoxins, PLA 2 , β-bungarotoxin, venom protein E2, frontoxin III, LAO and C-type lectin. The implications of our results for the production of Micrurus antivenoms are discussed.

Details

ISSN :
18767737
Volume :
74
Issue :
9
Database :
OpenAIRE
Journal :
Journal of proteomics
Accession number :
edsair.doi.dedup.....04ecc9b856d7df4bd346e8db64b7485d