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A traditional Chinese medicine formula inhibits tumor growth in mice and regulates the miR-34b/c-Met/β-catenin pathway
- Source :
- Journal of ethnopharmacology. 260
- Publication Year :
- 2020
-
Abstract
- Ethnopharmacology relevance Si-Jun-Zi-Tang (SJZT) is a traditional Chinese medicine formula used to treat chronic and debilitating diseases including melanoma. SJZT-based therapies have achieved good clinical outcomes in melanoma management. However, the pharmacological basis of SJZT for its clinical use in melanoma treatment is not fully understood. Aim of the study To investigate the anti-melanoma effects and mechanism of action of an ethanolic extract of SJZT. Materials and methods SJZT was extracted using 50% ethanol. A murine B16 melanoma-bearing mouse model was employed to investigate the anti-melanoma effects of SJZT. microRNA (miRNA) and mRNA levels were examined by RT-qPCR, and protein levels were measured by Western blotting. Results SJZT significantly inhibited B16 tumor growth in mice. Mechanistic investigations revealed that SJZT elevated miR-34b (a tumor suppressing miRNA), and lowered c-Met (a miR-34b target gene) and β-catenin (a downstream molecule of c-Met signaling) expression levels in the B16 tumors. Conclusions In this study we found, for the first time, that SJZT exerts anti-melanoma effects and regulates the miR-34b/c-Met/β-catenin pathway in a melanoma mouse model. Our findings provide pharmacological justifications for the clinical use of SJZT in treating melanoma.
- Subjects :
- Male
C-Met
Melanoma, Experimental
Traditional Chinese medicine
03 medical and health sciences
chemistry.chemical_compound
0302 clinical medicine
Drug Discovery
microRNA
medicine
Animals
Tumor growth
neoplasms
beta Catenin
030304 developmental biology
Cell Proliferation
Pharmacology
0303 health sciences
business.industry
Melanoma
Proto-Oncogene Proteins c-met
medicine.disease
Antineoplastic Agents, Phytogenic
Tumor Burden
Blot
Gene Expression Regulation, Neoplastic
Mice, Inbred C57BL
MicroRNAs
chemistry
Mechanism of action
030220 oncology & carcinogenesis
Catenin
Cancer research
medicine.symptom
business
Drugs, Chinese Herbal
Signal Transduction
Subjects
Details
- ISSN :
- 18727573
- Volume :
- 260
- Database :
- OpenAIRE
- Journal :
- Journal of ethnopharmacology
- Accession number :
- edsair.doi.dedup.....04e7229f2811ac12d2c6981f36ea5dcb