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Stable kinetochore–microtubule attachment is sufficient to silence the spindle assembly checkpoint in human cells
- Source :
- Nature Communications
- Publication Year :
- 2015
- Publisher :
- Springer Science and Business Media LLC, 2015.
-
Abstract
- During mitosis, duplicated sister chromatids attach to microtubules emanating from opposing sides of the bipolar spindle through large protein complexes called kinetochores. In the absence of stable kinetochore–microtubule attachments, a cell surveillance mechanism known as the spindle assembly checkpoint (SAC) produces an inhibitory signal that prevents anaphase onset. Precisely how the inhibitory SAC signal is extinguished in response to microtubule attachment remains unresolved. To address this, we induced formation of hyper-stable kinetochore–microtubule attachments in human cells using a non-phosphorylatable version of the protein Hec1, a core component of the attachment machinery. We find that stable attachments are sufficient to silence the SAC in the absence of sister kinetochore bi-orientation and strikingly in the absence of detectable microtubule pulling forces or tension. Furthermore, we find that SAC satisfaction occurs despite the absence of large changes in intra-kinetochore distance, suggesting that substantial kinetochore stretching is not required for quenching the SAC signal.<br />The spindle assembly checkpoint prevents mitotic progression when chromosomes are not properly attached to the mitotic spindle. Here Tauchman et al. show that stable microtubule attachment to the kinetochore, and not tension generated from this interaction, is sufficient to silence the checkpoint.
- Subjects :
- Mitosis
General Physics and Astronomy
Spindle Apparatus
Biology
Microtubules
Article
General Biochemistry, Genetics and Molecular Biology
Chromosome segregation
03 medical and health sciences
0302 clinical medicine
Microtubule
Chromosome Segregation
Humans
Sister chromatids
Kinetochores
030304 developmental biology
Anaphase
0303 health sciences
Multidisciplinary
Kinetochore
Nuclear Proteins
General Chemistry
Spindle apparatus
Cell biology
Cytoskeletal Proteins
Spindle checkpoint
M Phase Cell Cycle Checkpoints
030217 neurology & neurosurgery
HeLa Cells
Subjects
Details
- ISSN :
- 20411723
- Volume :
- 6
- Database :
- OpenAIRE
- Journal :
- Nature Communications
- Accession number :
- edsair.doi.dedup.....04df66ac403fe51180323b22d4433222