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Transient receptor potential melastatin 2 ion channel activity in ovarian hyperstimulation syndrome physiopathology
- Source :
- Turkish Journal of Medical Sciences
- Publication Year :
- 2021
- Publisher :
- The Scientific and Technological Research Council of Turkey (TUBITAK-ULAKBIM) - DIGITAL COMMONS JOURNALS, 2021.
-
Abstract
- Background/aim Ovarian hyperstimulation syndrome (OHSS) is a complication of ovarian stimulation with increased vascular endothelial growth factor (VEGF) and vascular permeability in the ovarian tissue. Transient receptor potential melastatin 2 (TRPM2) is known to be associated with angiogenesis and vascular permeability. In this experimental study, we aimed to investigate the activity of TRPM2 in the development of OHSS. Materials and methods Fourteen immature female rats were divided into two groups. Group 1 was the control group, and Group 2 was the OHSS group that was exposed to 10 IU of subcutaneous application of FSH for four days and 30 IU of human chorionic gonadotropin (hCG) on the 5th day. At the end of the experiment, the ovaries were removed. The right ovarian tissues were stored in 10% formol for histopathological and immunohistochemical examination. The left ovarian tissues were stored at –80 °C for biochemical examinations. VEGF, tumor necrosis factor-alpha (TNF‐α) and malondialdehyde (MDA) levels were measured in the ovarian tissue. Congestion, edema, apoptosis and TRPM2 immunoreactivity were evaluated. Results There was a significant increase in ovarian weight in the OHSS group compared to the control group. There was a significant increase in congestion, edema, apoptosis and TRPM2 immunoreactivity in the OHSS group. A significant increase in tissue levels of VEGF, TNF‐α and MDA was also found in the OHSS group compared to the control group. Conclusion As a result of our experiment, it was found that increased TRPM2 immunoreactivity on hyperstimulated rat ovary may be the reason or result of edema and congestion. Further studies are needed to discuss our results.
- Subjects :
- Vascular Endothelial Growth Factor A
endocrine system
Angiogenesis
TRPM Cation Channels
Ovarian hyperstimulation syndrome
Vascular permeability
Ovary
Article
Human chorionic gonadotropin
Andrology
angiogenesis
Ovarian Hyperstimulation Syndrome
chemistry.chemical_compound
Transient Receptor Potential Channels
Malondialdehyde
Edema
Animals
Medicine
TRPM2
Tumor Necrosis Factor-alpha
business.industry
General Medicine
medicine.disease
VEGF
Pathophysiology
Rats
Vascular endothelial growth factor
medicine.anatomical_structure
chemistry
OHSS
Rat
Female
medicine.symptom
business
Subjects
Details
- ISSN :
- 13036165
- Volume :
- 51
- Database :
- OpenAIRE
- Journal :
- TURKISH JOURNAL OF MEDICAL SCIENCES
- Accession number :
- edsair.doi.dedup.....04da2424d29fdb402a1966dd6e67cda8