Protein kinase Cθ gene expression is oppositely regulated by GCN5 and EBF1 in immature B cells

In this study, we revealed that GCN5 and early B cell factor 1 (EBF1) participate in regulation of protein kinase Cθ (PKCθ) gene expression in an opposite manner in immature B cells. GCN5-deficiency in DT40 caused drastic down-regulation of transcription of PKCθ. In contrast, EBF1-deficiency brought about remarkable up-regulation of that of PKCθ, and re-expression of EBF1 dramatically suppressed transcription of PKCθ. Chromatin immunoprecipitation assay revealed that GCN5 binds to the 5′-flanking region of the chicken PKCθ gene and acetylates histone H3, and EBF1 binds to the 5′-flanking region of the gene surrounding putative EBF1 binding motifs.