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Genome-Wide Fitness Analyses of the Foodborne Pathogen Campylobacter jejuni in In Vitro and In Vivo models
- Source :
- de Vries, S P W, Gupta, S, Baig, A, Wright, E, Wedley, A, Jensen, A N, Lora, L L, Humphrey, S, Skovgård, H, Macleod, K, Pont, E, Wolanska, D P, L'Heureux, J, Mobegi, F M, Smith, D G E, Everest, P, Zomer, A, Williams, N, Wigley, P, Humphrey, T, Maskell, D J & Grant, A J 2017, ' Genome-Wide Fitness Analyses of the Foodborne Pathogen Campylobacter jejuni in In Vitro and In Vivo models ', Scientific Reports, vol. 7, 1251 . https://doi.org/10.1101/085720, Scientific Reports, Scientific Reports, Vol 7, Iss 1, Pp 1-17 (2017), de Vries, S P W, Gupta, S, Baig, A, Wright, E, Wedley, A, Jensen, A N, Lora, L L, Humphrey, S, Skovgard, H, Macleod, K, Pont, E, Wolanska, D P, L'Heureux, J, Mobegi, F M, Smith, D G E, Everest, P, Zomer, A, Williams, N, Wigley, P, Humphrey, T, Maskell, D J & Grant, A J 2017, ' Genome-wide fitness analyses of the foodborne pathogen Campylobacter jejuni in in vitro and in vivo models ', Scientific Reports, vol. 7, 1251 . https://doi.org/10.1038/s41598-017-01133-4
- Publication Year :
- 2017
-
Abstract
- Infection byCampylobacteris recognised as the most common cause of foodborne bacterial illness worldwide. Faecal contamination of meat, especially chicken, during processing represents a key route of transmission to humans. There is currently no licenced vaccine and noCampylobacter-resistant chickens. In addition, preventative measures aimed at reducing environmental contamination and exposure of chickens toCampylobacter jejuni(biosecurity) have been ineffective. There is much interest in the factors/mechanisms that driveC. jejunicolonisation and infection of animals, and survival in the environment. It is anticipated that understanding these mechanisms will guide the development of effective intervention strategies to reduce the burden ofC. jejuniinfection. Here we present a comprehensive analysis ofC. jejunifitness during growth and survival within and outside hosts. A comparative analysis of transposon (Tn) gene inactivation libraries in threeC. jejunistrains by Tn-seq demonstrated that a large proportion, 331 genes, of theC. jejunigenome is dedicated to (in vitro) growth. An extensive Tn library inC. jejuniM1cam (~10,000 mutants) was screened for the colonisation of commercial broiler chickens, survival in houseflies and under nutrient-rich and–poor conditions at low temperature, and infection of human gut epithelial cells. We reportC. jejunifactors essential throughout its life cycle and we have identified genes that fulfil important roles across multiple conditions, includingmaf3, fliW, fliD, pflBandcapM, as well as novel genes uniquely implicated in survival outside hosts. Taking a comprehensive screening approach has confirmed previous studies, that the flagella are central to the ability ofC. jejunito interact with its hosts. Future efforts should focus on how to exploit this knowledge to effectively control infections caused byC. jejuni.Author SummaryCampylobacter jejuniis the leading bacterial cause of human diarrhoeal disease.C. jejuniencounters and has to overcome a wide range of “stress” conditions whilst passing through the gastrointestinal tract of humans and other animals, during processing of food products, on/in food and in the environment. We have taken a comprehensive approach to understand the basis ofC. jejunigrowth and within/outside host survival, with the aim to inform future development of intervention strategies. Using a genome-wide transposon gene inactivation approach we identified genes core to the growth ofC. jejuni. We also determined genes that were required during the colonisation of chickens, survival in the housefly and under nutrient-rich and –poor conditions at low temperature, and during interaction with human gut epithelial tissue culture cells. This study provides a comprehensive dataset linkingC. jejunigenes to growth and survival in models relevant to its life cycle. Genes important across multiple models were identified as well as genes only required under specific conditions. We identified that a large proportion of theC. jejunigenome is dedicated to growth and that the flagella fulfil a prominent role in the interaction with hosts. Our data will aid development of effective control strategies.
- Subjects :
- Transposable element
Science
Biosecurity
Biology
medicine.disease_cause
Genome
Campylobacter jejuni
Article
Microbiology
Cell Line
03 medical and health sciences
SDG 3 - Good Health and Well-being
Houseflies
Campylobacter Infections
medicine
Animals
Humans
Gene
030304 developmental biology
0303 health sciences
Microbial Viability
030306 microbiology
Transmission (medicine)
Campylobacter
Gene Expression Profiling
Temperature
Epithelial Cells
biology.organism_classification
3. Good health
Culture Media
Colonisation
Mutagenesis, Insertional
Host-Pathogen Interactions
Medicine
Genetic Fitness
Chickens
Genome, Bacterial
Subjects
Details
- Language :
- English
- ISSN :
- 20452322
- Database :
- OpenAIRE
- Journal :
- de Vries, S P W, Gupta, S, Baig, A, Wright, E, Wedley, A, Jensen, A N, Lora, L L, Humphrey, S, Skovgård, H, Macleod, K, Pont, E, Wolanska, D P, L'Heureux, J, Mobegi, F M, Smith, D G E, Everest, P, Zomer, A, Williams, N, Wigley, P, Humphrey, T, Maskell, D J & Grant, A J 2017, ' Genome-Wide Fitness Analyses of the Foodborne Pathogen Campylobacter jejuni in In Vitro and In Vivo models ', Scientific Reports, vol. 7, 1251 . https://doi.org/10.1101/085720, Scientific Reports, Scientific Reports, Vol 7, Iss 1, Pp 1-17 (2017), de Vries, S P W, Gupta, S, Baig, A, Wright, E, Wedley, A, Jensen, A N, Lora, L L, Humphrey, S, Skovgard, H, Macleod, K, Pont, E, Wolanska, D P, L'Heureux, J, Mobegi, F M, Smith, D G E, Everest, P, Zomer, A, Williams, N, Wigley, P, Humphrey, T, Maskell, D J & Grant, A J 2017, ' Genome-wide fitness analyses of the foodborne pathogen Campylobacter jejuni in in vitro and in vivo models ', Scientific Reports, vol. 7, 1251 . https://doi.org/10.1038/s41598-017-01133-4
- Accession number :
- edsair.doi.dedup.....04cd98d88ed69c72e95f267cbebe4cfe