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RNA-seq of spinal cord from nerve-injured rats after spinal cord stimulation
- Source :
- Molecular Pain
- Publication Year :
- 2018
-
Abstract
- Spinal cord stimulation has become an important modality in pain treatment especially for neuropathic pain conditions refractory to pharmacotherapy. However, the molecular control of inhibitory and excitatory mechanisms observed after spinal cord stimulation are poorly understood. Here, we used RNA-seq to identify differences in the expression of genes and gene networks in spinal cord tissue from nerve-injured rats with and without repetitive conventional spinal cord stimulation treatment. Five weeks after chronic constrictive injury to the left sciatic nerve, male and female rats were randomized to receive repetitive spinal cord stimulation or no treatment. Rats receiving spinal cord stimulation underwent epidural placement of a miniature stimulating electrode and received seven sessions of spinal cord stimulation (50 Hz, 80% motor threshold, 0.2 ms, constant current bipolar stimulation, 120 min/session) over four consecutive days. Within 2 h after the last spinal cord stimulation treatment, the L4-L6 spinal segments ipsilateral to the side of nerve injury were harvested and used to generate libraries for RNA-seq. Our RNA-seq data suggest further increases of many existing upregulated immune responses in chronic constrictive injury rats after repetitive spinal cord stimulation, including transcription of cell surface receptors and activation of non-neuronal cells. We also demonstrate that repetitive spinal cord stimulation represses transcription of several key synaptic signaling genes that encode scaffold proteins in the post-synaptic density. Our transcriptional studies suggest a potential relationship between specific genes and the therapeutic effects observed in patients undergoing conventional spinal cord stimulation after nerve injury. Furthermore, our results may help identify new therapeutic targets for improving the efficacy of conventional spinal cord stimulation and other chronic pain treatments.
- Subjects :
- Male
Down-Regulation
Constriction, Pathologic
Inhibitory postsynaptic potential
Models, Biological
Rats, Sprague-Dawley
03 medical and health sciences
Cellular and Molecular Neuroscience
0302 clinical medicine
030202 anesthesiology
Medicine
Animals
pain
Sex Characteristics
Spinal Cord Stimulation
business.industry
Sequence Analysis, RNA
Gene Expression Profiling
Therapeutic effect
Chronic pain
spinal cord
Nerve injury
medicine.disease
Spinal cord
Sciatic Nerve
3. Good health
Up-Regulation
Anesthesiology and Pain Medicine
medicine.anatomical_structure
Gene Ontology
Anesthesia
Neuropathic pain
Chronic Disease
Synapses
Excitatory postsynaptic potential
gene expression
Molecular Medicine
Neuralgia
Female
Synaptic signaling
nerve injury
medicine.symptom
RNA-seq
business
030217 neurology & neurosurgery
Research Article
Subjects
Details
- ISSN :
- 17448069
- Volume :
- 14
- Database :
- OpenAIRE
- Journal :
- Molecular pain
- Accession number :
- edsair.doi.dedup.....04c70dab2dc4941a6a1853ffc9594b49