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Molecular detection of Yersinia pestis isolates of Indian origin by using Pla specific monoclonal antibodies
- Source :
- Comparative Immunology, Microbiology and Infectious Diseases. 28:131-144
- Publication Year :
- 2005
- Publisher :
- Elsevier BV, 2005.
-
Abstract
- Monoclonal antibodies (MAbs) were generated against the recombinant plasminogen activator (Pla) protein of Yersinia pestis. These MAbs detected Pla in all the 18 isolates of Y. pestis obtained from the sputum of pneumonic plague patients and from the liver and spleen of rodents from plague-affected areas of India during 1994–1995 as well as in seven of the eight isolates obtained from rodents in the surveillance regions of Hosur and Palmner in India during 1998 by simple dot-ELISA. In immunoblotting, the MAbs reacted with the Pla antigen only in Y. pestis isolates at 37 and 35 kDa region. These monoclonal antibodies, being strictly specific, can be used for detecting Y. pestis isolates that are Fraction 1 antigen-negative. Also, the radiolabelled pla fragment hybridized specifically to the representative DNA samples of Y. pestis isolates.
- Subjects :
- DNA, Bacterial
Pneumonic plague
Yersinia pestis
medicine.drug_class
Blotting, Western
Immunology
India
Enzyme-Linked Immunosorbent Assay
Monoclonal antibody
Polymerase Chain Reaction
Microbiology
Disease Outbreaks
law.invention
Mice
Plasminogen Activators
Antigen
law
Escherichia coli
medicine
Animals
Humans
Immunology and Allergy
Cloning, Molecular
Mice, Inbred BALB C
Plague
General Veterinary
biology
Hybridization probe
Antibodies, Monoclonal
Nucleic Acid Hybridization
General Medicine
biology.organism_classification
medicine.disease
Virology
Enterobacteriaceae
Recombinant Proteins
Infectious Diseases
Recombinant DNA
Rabbits
Plasminogen activator
Plasmids
Subjects
Details
- ISSN :
- 01479571
- Volume :
- 28
- Database :
- OpenAIRE
- Journal :
- Comparative Immunology, Microbiology and Infectious Diseases
- Accession number :
- edsair.doi.dedup.....04af2ed2168c26f2188a0f6b94ea5128