Additional file 2 of Na+/Ca2+ exchanger isoform 1 takes part to the Ca2+-related prosurvival pathway of SOD1 in primary motor neurons exposed to beta-methylamino-l-alanine

Additional file 1. (A) Immunolocalization of NCX1 (a,d) and MAP2 (b,e) within a motor-neuron enriched culture under control conditions. Nuclei were stained with nuclear DNA stain 4, 6-diamino-2-phenylinndole (DAPI). Arrows indicate MAP2-positive cells with higher level of NCX1 expression. (B) Immunolocalization of NCX1 and NCX3 in differentiated NSC-34 cells. (C) Quantification of SOD1-induced [Ca2+]i in presence of CNQX (20 ��M), MK801 (10 ��M), or CB-DMB (1 ��M) in motor neurons expressed as ���% of increase. All the experiments were repeated at least three times on at least 35 cells for each group; *p < 0.001 vs control (basal values of [Ca2+]i) .