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Downregulation of circ_008018 protects against cerebral ischemia–reperfusion injury by targeting miR-99a
- Source :
- Biochemical and Biophysical Research Communications. 499:758-764
- Publication Year :
- 2018
- Publisher :
- Elsevier BV, 2018.
-
Abstract
- Circular RNAs (circRNAs) are highly expressed in eukaryotic cells and regulate physiological and pathophysiological processes. However, the role of circRNAs in cerebral ischemia–reperfusion (I/R) injury remains largely unknown. In this study, we found that circ_008018 level was higher in the cortical tissue of mice with middle cerebral artery occlusion as compared to those in the sham group 24 h after reperfusion. Knockdown of circ_008018 attenuated cerebral I/R-induced brain tissue damage and neurological deficits in mice by inducing microRNA miR-99a overexpression. The decreased phosphorylation of Akt and glycogen synthase kinase 3β caused by I/R was partly reversed by circ_008018 silencing or miR-99a overexpression. Taken together, these results provide new insight into the mechanisms of apoptosis resulting from cerebral I/R injury and suggest that targeted inhibition of circ_008018 can protect against subsequent neurological damage.
- Subjects :
- Male
0301 basic medicine
Biophysics
Ischemia
Down-Regulation
Biochemistry
Brain Ischemia
Phosphatidylinositol 3-Kinases
03 medical and health sciences
Downregulation and upregulation
GSK-3
microRNA
Animals
Medicine
Gene silencing
Gene Silencing
Molecular Biology
Protein kinase B
Gene knockdown
Glycogen Synthase Kinase 3 beta
Base Sequence
business.industry
RNA, Circular
Cell Biology
medicine.disease
Mice, Inbred C57BL
MicroRNAs
030104 developmental biology
Reperfusion Injury
Cancer research
RNA
business
Proto-Oncogene Proteins c-akt
Reperfusion injury
Signal Transduction
Subjects
Details
- ISSN :
- 0006291X
- Volume :
- 499
- Database :
- OpenAIRE
- Journal :
- Biochemical and Biophysical Research Communications
- Accession number :
- edsair.doi.dedup.....046f29078cba32cd2d702e3790319257