Back to Search
Start Over
Leishmania amazonensis promastigotes evade complement killing by interfering with the late steps of the cascade
- Source :
- Parasitology. 115:601-609
- Publication Year :
- 1997
- Publisher :
- Cambridge University Press (CUP), 1997.
-
Abstract
- During their growth in vitro, promastigotes of Leishmania amazonensis undergo differentiation from complement-susceptible to complement-resistant forms. Here, we demonstrate that both forms bind comparable amounts of C3 on their surfaces, with the predominant molecule species being the haemolytically active C3b. Likewise, equivalent amounts of C9 are deposited on both forms of promastigotes. However, while C9-bearing complexes are exposed on the cell surface of resistant promastigotes, they are cryptic in the susceptible stage of the parasites. The membrane fraction of complement-resistant promastigote lysates has the ability to inhibit complement-mediated haemolysis, blocking C9, but not C3 deposition to complement-activating complexes. Moreover, the membrane fraction of complement-resistant promastigote lysates can inhibit the late steps of guinea-pig erythrocyte lysis much more efficiently than complement-susceptible ones. Our results indicate that L. amazonensis promastigotes evade complement killing by inhibiting the cytolytic pathway of the complement cascade.
- Subjects :
- Complement Pathway, Alternative
Guinea Pigs
Leishmania mexicana
Cell
Enzyme-Linked Immunosorbent Assay
Biology
Complement Hemolytic Activity Assay
Host-Parasite Interactions
Microbiology
medicine
Animals
Humans
Complement Inactivator Proteins
Leishmania amazonensis
Cell Cycle
Cell Membrane
Kinetoplastida
Complement C3
Complement System Proteins
Complement C9
Haemolysis
biology.organism_classification
In vitro
Cell biology
Complement system
Cytolysis
Infectious Diseases
medicine.anatomical_structure
Complement C3b
Protozoa
Animal Science and Zoology
Parasitology
Subjects
Details
- ISSN :
- 14698161 and 00311820
- Volume :
- 115
- Database :
- OpenAIRE
- Journal :
- Parasitology
- Accession number :
- edsair.doi.dedup.....0451e0920f7216329716aba636f92fea