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The Neurokinin-1 Receptor Is a Target in Pediatric Rhabdoid Tumors
- Source :
- Current Oncology; Volume 29; Issue 1; Pages: 94-110, Current Oncology, Vol 29, Iss 8, Pp 94-110 (2022), Curr. Oncol. 29, 94-110 (2022), Current Oncology
- Publication Year :
- 2021
- Publisher :
- Multidisciplinary Digital Publishing Institute, 2021.
-
Abstract
- Rhabdoid tumors (RT) are among the most aggressive tumors in early childhood. Overall survival remains poor, and treatment only effectively occurs at the cost of high toxicity and late adverse effects. It has been reported that the neurokinin-1 receptor/ substance P complex plays an important role in cancer and proved to be a promising target. However, its role in RT has not yet been described. This study aims to determine whether the neurokinin-1 receptor is expressed in RT and whether neurokinin-1 receptor (NK1R) antagonists can serve as a novel therapeutic approach in treating RTs. By in silico analysis using the cBio Cancer Genomics Portal we found that RTs highly express neurokinin-1 receptor. We confirmed these results by RT-PCR in both tumor cell lines and in human tissue samples of various affected organs. We demonstrated a growth inhibitory and apoptotic effect of aprepitant in viability assays and flow cytometry. Furthermore, this effect proved to remain when used in combination with the cytostatic cisplatin. Western blot analysis showed an upregulation of apoptotic signaling pathways in rhabdoid tumors when treated with aprepitant. Overall, our findings suggest that NK1R may be a promising target for the treatment of RT in combination with other anti-cancer therapies and can be targeted with the NK1R antagonist aprepitant.
- Subjects :
- substance P
NK-1 receptor
apoptosis
Neoplasms. Tumors. Oncology. Including cancer and carcinogens
Receptors, Neurokinin-1
NK-1 receptor antagonist
Article
Neurokinin-1 Receptor Antagonists
Child, Preschool
Humans
Apoptosis
Cancer
Nk-1 Receptor
Nk-1 Receptor Antagonist
Rhabdoid Tumor
Substance P
cancer
Child
rhabdoid tumor
Aprepitant
RC254-282
Cell Proliferation
Subjects
Details
- Language :
- English
- ISSN :
- 17187729
- Database :
- OpenAIRE
- Journal :
- Current Oncology; Volume 29; Issue 1; Pages: 94-110
- Accession number :
- edsair.doi.dedup.....044f3171f8934797cf9d19adb7549518
- Full Text :
- https://doi.org/10.3390/curroncol29010008