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Simple workflow to repurpose SARS-CoV-2 swab/serum samples for the isolation of cost-effective antibody/antigens for proteotyping applications and diagnosis
- Source :
- Analytical and Bioanalytical Chemistry
- Publication Year :
- 2021
- Publisher :
- Springer Science and Business Media LLC, 2021.
-
Abstract
- Supply shortage for the development and production of preventive, therapeutic, and diagnosis tools during the COVID-19 pandemic is an important issue affecting the wealthy and poor nations alike. Antibodies and antigens are especially needed for the production of immunological-based testing tools such as point-of-care tests. Here, we propose a simple and quick magnetic nanoparticle (MNP)-based separation/isolation approach for the repurposing of infected human samples to produce specific antibodies and antigen cocktails. Initially, an antibody cocktail was purified from serums via precipitation and immunoaffinity chromatography. Purified antibodies were conjugated onto MNPs and used as an affinity matrix to separate antigens. The characterization process was performed by ELISA, SDS-PAGE, electrochemistry, isothermal titration calorimetry, and LC-Q-TOF-MS/MS analyses. The MNP-separated peptides can be used for mass spectrometry-based as well as paper-based lateral flow assay diagnostic. The exploitation of the current workflow for the development of efficient diagnostic tools, specific treatments, and fundamental research can significantly impact the present or eventual pandemic. This workflow can be considered as a two birds, one stone-like strategy.<br />TUBITAK [120R021]; Republic of Turkey Ministry of Development [2010K120810/2020K12150700, 2016K121190]<br />The study was supported by TUBITAK (Project number: 120R021). The Republic of Turkey Ministry of Development provided the support for the infrastructure of EGEMATAL (Ege University/Izmir) via 2010K120810/2020K12150700 and 2016K121190 grants.
- Subjects :
- Coronavirus disease 2019 (COVID-19)
Antigen/antibody purification
Cost-Benefit Analysis
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)
Enzyme-Linked Immunosorbent Assay
Lateral flow assay
Calorimetry
Antibodies, Viral
Tandem mass spectrometry
Biochemistry
Antibodies
Specimen Handling
Workflow
Analytical Chemistry
Antigen
Affinity chromatography
Tandem Mass Spectrometry
Information
Humans
Magnetic nanoparticle (MNP)–based separation
Viremia
Antigens, Viral
Purification
Cleavage
Immunoassay
Magnetic nanoparticle (MNP)-based separation
antibody purification
Mass spectrometry
biology
SARS-CoV-2
Chemistry
COVID-19
Magnetic Nanoparticles
Isolation (microbiology)
Virology
biology.protein
Electrophoresis, Polyacrylamide Gel
Antibody
Research Paper
Subjects
Details
- ISSN :
- 16182650 and 16182642
- Volume :
- 413
- Database :
- OpenAIRE
- Journal :
- Analytical and Bioanalytical Chemistry
- Accession number :
- edsair.doi.dedup.....04318aee1dcd639e689bfa8e4bb51f8a