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Human rotavirus specific T cells: quantification by ELISPOT and expression of homing receptors on CD4+ T cells
- Source :
- Virology. (2):671-679
- Publisher :
- Elsevier Inc.
-
Abstract
- Using an intracellular cytokine assay, we recently showed that the frequencies of rotavirus (RV)-specific CD4(+) and CD8(+) T cells secreting INFgamma, circulating in RV infected and healthy adults, are very low compared to the frequencies of circulating cytomegalovirus (CMV) reactive T cells in comparable individuals. In children with acute RV infection, these T cells were barely or not detectable. In the present study, an ELISPOT assay enabled detection of circulating RV-specific INFgamma-secreting cells in children with RV diarrhea but not in children with non-RV diarrhea without evidence of a previous RV infection. Using microbead-enriched CD4(+) and CD8(+) T cell subsets, IFNgamma-secreting RV-specific CD8(+) but not CD4(+) T cells were detected in recently infected children. Using the same approach, both CD4(+) and CD8(+) RV-specific T cells were detected in healthy adults. Furthermore, stimulation of purified subsets of PBMC that express lymphocyte homing receptors demonstrated that RV-specific INFgamma-secreting CD4(+) T cells from adult volunteers preferentially express the intestinal homing receptor alpha4beta7, but not the peripheral lymph node homing receptor L-selectin. In contrast, CMV-specific INFgamma-secreting CD4(+) T cells preferentially express L-selectin but not alpha4beta7. These results suggest that the expression of homing receptors on virus-specific T cells depends on the organ where these cells were originally stimulated and that their capacity to secrete INFgamma is independent of the expression of these homing receptors.
- Subjects :
- Adult
CD4-Positive T-Lymphocytes
Rotavirus
Diarrhea
T cell
Receptors, Lymphocyte Homing
T cells
Enzyme-Linked Immunosorbent Assay
Biology
CD8-Positive T-Lymphocytes
Lymphocyte Activation
Rotavirus Infections
03 medical and health sciences
Interleukin 21
Interferon-gamma
0302 clinical medicine
Virology
medicine
Cytotoxic T cell
Humans
IL-2 receptor
Antigen-presenting cell
Lymphocyte homing receptor
Child
Children
030304 developmental biology
Interleukin 3
0303 health sciences
Infant
Middle Aged
Natural killer T cell
3. Good health
medicine.anatomical_structure
Child, Preschool
Immunology
Lymphocyte homing receptors
Cytokines
Interleukin-4
030215 immunology
Subjects
Details
- Language :
- English
- ISSN :
- 00426822
- Issue :
- 2
- Database :
- OpenAIRE
- Journal :
- Virology
- Accession number :
- edsair.doi.dedup.....04250a66ca25eda23792709a2fff354b
- Full Text :
- https://doi.org/10.1016/S0042-6822(03)00507-5