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Antibodies reactive with native lysozyme elicited by a completely synthetic antigen
- Source :
- Proceedings of the National Academy of Sciences of the United States of America. 68(7)
- Publication Year :
- 1971
-
Abstract
- A synthetic peptide consisting of the aminoacid sequence of residues 64-82 of lysozyme, with alanine replacing cysteine as residue 76, was prepared by the solid-phase technique. Mild reduction followed by reoxidation in air of the deprotected peptide led to the formation of a closed loop containing an intrachain disulfide bond. A conjugate consisting of this “loop” attached to multi-poly(DL-alanyl)-poly(L-lysine) elicited, in rabbits and goats, the formation of antibodies capable of reacting with lysozyme and with the loop peptide prepared from it. These immunological interactions can be inhibited by either lysozyme or the loop peptide, but not by the performic acid-oxidized open-chain peptide. Thus, the antibodies elicited by the completely synthetic antigen show specificity toward the “loop” structure (residues 64-80) of native lysozyme.
- Subjects :
- Electrophoresis
Paper
Chemical Phenomena
Synthetic antigen
Peptide
Cross Reactions
Naphthalenes
Antibodies
Antigen-Antibody Reactions
chemistry.chemical_compound
Residue (chemistry)
Polysaccharides
Iodine Isotopes
medicine
Animals
Bacteriophages
Fluorometry
Trypsin
Cysteine
Amino Acids
Alanine
chemistry.chemical_classification
Carbon Isotopes
Multidisciplinary
Goats
Amino acid
Molecular Weight
Chemistry
chemistry
Biochemistry
Energy Transfer
Muramidase
Rabbits
Biological Sciences: Biochemistry
Lysozyme
Sulfonic Acids
Peptides
Oxidation-Reduction
Ultracentrifugation
medicine.drug
Subjects
Details
- ISSN :
- 00278424
- Volume :
- 68
- Issue :
- 7
- Database :
- OpenAIRE
- Journal :
- Proceedings of the National Academy of Sciences of the United States of America
- Accession number :
- edsair.doi.dedup.....0404ef94bbd80036888c22f7417ec91b