Back to Search
Start Over
The Ebola Virus VP24 Protein Prevents hnRNP C1/C2 Binding to Karyopherin α1 and Partially Alters its Nuclear Import
- Publication Year :
- 2011
- Publisher :
- Oxford University Press, 2011.
-
Abstract
- The Ebola virus (EBOV) protein VP24 inhibits type I and II interferon (IFN) signaling by binding to NPI-1 subfamily karyopherin α (KPNA) nuclear import proteins, preventing their interaction with tyrosine-phosphorylated STAT1 (phospho-STAT1). This inhibits phospho-STAT1 nuclear import. A biochemical screen now identifies heterogeneous nuclear ribonuclear protein complex C1/C2 (hnRNP C1/C2) nuclear import as an additional target of VP24. Co-immunoprecipitation studies demonstrate that hnRNP C1/C2 interacts with multiple KPNA family members, including KPNA1. Interaction with hnRNP C1/C2 occurs through the same KPNA1 C-terminal region (amino acids 424-457) that binds VP24 and phospho-STAT1. The ability of hnRNP C1/C2 to bind KPNA1 is diminished in the presence of VP24, and cells transiently expressing VP24 redistribute hnRNP C1/C2 from the nucleus to the cytoplasm. These data further define the mechanism of hnRNP C1/C2 nuclear import and demonstrate that the impact of EBOV VP24 on nuclear import extends beyond STAT1.
- Subjects :
- alpha Karyopherins
Cytoplasm
Immunoprecipitation
viruses
Active Transport, Cell Nucleus
Plasma protein binding
Biology
environment and public health
Mice
Viral Proteins
Protein structure
Interferon
Chlorocebus aethiops
medicine
Immunology and Allergy
Viral Biology
Animals
Humans
Vero Cells
Karyopherin
chemistry.chemical_classification
Cell Nucleus
Heterogeneous-Nuclear Ribonucleoprotein Group C
Ebolavirus
Molecular biology
Protein Structure, Tertiary
Cell nucleus
Infectious Diseases
medicine.anatomical_structure
HEK293 Cells
chemistry
Gene Expression Regulation
Rabbits
Nuclear transport
medicine.drug
Protein Binding
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Accession number :
- edsair.doi.dedup.....03ffcf77f2837b0327feda2e972eead6