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New insights on the utilization of ultrasonicated mustard seed cake: chemical composition and antagonistic potential for root-knot nematode, Meloidogyne javanica
- Source :
- J Zhejiang Univ Sci B
- Publication Year :
- 2021
- Publisher :
- Zhejiang University Press, 2021.
-
Abstract
- This study focused, for the first time, on the effect of ultrasonic features on the extraction efficiency of secondary metabolites in mustard seed cake (MSC). The nematostatic potential of sonicated seed cake was examined against the second-stage juveniles (J2s) of root-knot nematode, Meloidogyne javanica. The results show that a 35 ppm (parts per million) concentration of a sonicated extract (SE) sample of MSC caused 65% J2s mortality at 18 h exposure period in vitro. It also significantly suppressed the root-knot index (RKI=0.94) in tomato roots. The lethal concentration values for SE were 51.76, 29.79, and 13.34 ppm, respectively, at 6, 12, and 18 h of the exposure period, and the lethal concentration values for the non-sonicated extract (NSE) sample were 116.95, 76.38, and 55.59 ppm, respectively, at similar exposure time. Sinapine and gluconapin were identified as the major compounds in ultrasonic-assisted MSC. Because of the high extraction efficiency of metabolites in the SE, all treatments of SE were shown to be antagonistic to J2s. Thus, this study of ultrasonication activity-based profiling of MSC may help generate target-based compounds at a scale relevant to the control of disease caused by nematodes in economic crops.
- Subjects :
- Crops, Agricultural
Spectrometry, Mass, Electrospray Ionization
food.ingredient
Sonication
Glucosinolates
Plant Roots
General Biochemistry, Genetics and Molecular Biology
Choline
chemistry.chemical_compound
food
Solanum lycopersicum
Sinapine
Root-knot nematode
Animals
Ultrasonics
Tylenchoidea
General Pharmacology, Toxicology and Pharmaceutics
Chemical composition
General Veterinary
biology
Chemistry
Extraction (chemistry)
General Medicine
Mustard seed
biology.organism_classification
Horticulture
Nematode
Seeds
Microscopy, Electron, Scanning
Solvents
Meloidogyne javanica
Research Article
Chromatography, Liquid
Mustard Plant
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Journal :
- J Zhejiang Univ Sci B
- Accession number :
- edsair.doi.dedup.....03fcc87809f2916d3991db293b7128cc