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Cellular localization of a plant protein PSAM 1 in arbuscular mycorrhizas of Pisum sativum
- Source :
- Planta, Planta, Springer Verlag, 1998, pp.153-157
- Publication Year :
- 1998
- Publisher :
- HAL CCSD, 1998.
-
Abstract
- Psam 1 is a single-copy gene which is activated during early plant-fungal interaction in wild-type pea inoculated with Glomus mosseae and which codes for PSAM 1, a putative protein of 108 amino acids. A synthetic peptide was designed of 108 amino acids. A synthetic peptide was designed in an antigenic region of this protein to produce a polyclonal antibody against PSAM 1 and to investigate its cellular localization. Western blot analysis revealed that a polypeptide of about 14.5 kDa accumulated more in mycorrhizal than non-mycorrhizal pea roots. The PSAM 1 antigen was immunolocated in planta in arbuscule-containing cells of mycorrhizal roots and especially in the cytoplasm surrounding young arbuscules in cortical cells, which suggests that its accumulation is somehow related to the symbiotic state of these cells.
- Subjects :
- 0106 biological sciences
Plant Science
01 natural sciences
Pisum
[SDV.GEN.GPL]Life Sciences [q-bio]/Genetics/Plants genetics
03 medical and health sciences
Western blot
[SDV.GEN.GPL] Life Sciences [q-bio]/Genetics/Plants genetics
Botany
Genetics
medicine
Animals
Mycorrhiza
Cellular localization
Glomus
ComputingMilieux_MISCELLANEOUS
Plant Proteins
030304 developmental biology
chemistry.chemical_classification
0303 health sciences
medicine.diagnostic_test
biology
Peas
Membrane Proteins
food and beverages
biology.organism_classification
Amino acid
Biochemistry
chemistry
Cytoplasm
Plant protein
Rabbits
010606 plant biology & botany
Subjects
Details
- Language :
- English
- ISSN :
- 00320935 and 14322048
- Database :
- OpenAIRE
- Journal :
- Planta, Planta, Springer Verlag, 1998, pp.153-157
- Accession number :
- edsair.doi.dedup.....03e83f516c50bcf75faa8db92d4fc6ef