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Evaluation of the filter paper blood collection method for detecting Og4C3 circulating antigen in bancroftian filariasis
- Source :
- Transactions of the Royal Society of Tropical Medicine and Hygiene. 92:407-410
- Publication Year :
- 1998
- Publisher :
- Oxford University Press (OUP), 1998.
-
Abstract
- Serological diagnosis of filariasis is generally known to be more reliable than detection of microfilariae. The recently developed Og4C3 enzyme-linked immunosorbent assay (ELISA) for detecting Wuchereria bancrofti circulating antigen has been shown to be very sensitive in diagnosing filiariasis using serum samples. The commercially available form of this ELISA, using whole blood collected on filter paper, has not been validated independently. We evaluated the sensitivity of this new method against standard 20 microL night blood films in 1808 paired samples from 18 communities in different endemic areas of Ghana. The diagnostic performance of the method was consistently low in all but 2 communities (sensitivity = 50.3%). This method of diagnosing filariasis is not suitable for field use in its present form.
- Subjects :
- Male
Circulating antigen
Enzyme-Linked Immunosorbent Assay
Rural Health
Biology
medicine.disease_cause
Ghana
Microfilaria
Serology
Filariasis
Age Distribution
Antigen
parasitic diseases
medicine
Animals
Humans
Wuchereria bancrofti
Whole blood
Blood Specimen Collection
Filter paper
Public Health, Environmental and Occupational Health
General Medicine
medicine.disease
Virology
Infectious Diseases
Antigens, Helminth
Immunology
Female
Parasitology
Subjects
Details
- ISSN :
- 00359203
- Volume :
- 92
- Database :
- OpenAIRE
- Journal :
- Transactions of the Royal Society of Tropical Medicine and Hygiene
- Accession number :
- edsair.doi.dedup.....03e6089fdf7a7b335756f6ec2c607843