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Salvianolic acid B improved insulin resistance through suppression of hepatic ER stress in ob/ob mice
- Source :
- Biochemical and biophysical research communications. 526(3)
- Publication Year :
- 2020
-
Abstract
- Impaired insulin sensitivity of insulin-sensitive tissues plays a major role in the pathogenesis of type 2 diabetes, salvianolic acid B (SalB), a natural antioxidant usually treated various cardiovascular diseases was also reported potential utility on diabetes and dyslipidemia. Based on these, we aimed to explored whether the antioxidant effect of SalB play a pivotal role in the molecular mechanisms leading to insulin resistance. We found that SalB improved glucose tolerance and insulin sensitivity, decreased serum ALT, AST and ALP levels of ob/ob mice. Also, transcription of Bip and CHOP, phosphorylation of PERK and IRE1 for endoplasmic reticulum stress (ER) and phosphorylation of IRS-1 for insulin sensitivity in the liver of ob/ob mice were relieved by SalB. Further, SalB decreased phosphorylation of PERK, IRE1 and IRS1 and transcription of Bip and CHOP stimulated by palmitate of hepatic cells HL7702, but did not reversed phosphorylation of JNK and IRS1 and transcription of Bip and CHOP when ER stress was stimulated by tunicamycin. These data shows that SalB improved insulin resistance of ob/ob mice through suppression of hepatic ER stress.
- Subjects :
- 0301 basic medicine
Male
medicine.medical_specialty
genetic structures
Biophysics
Palmitates
macromolecular substances
CHOP
Protein Serine-Threonine Kinases
Biochemistry
03 medical and health sciences
chemistry.chemical_compound
Mice
eIF-2 Kinase
0302 clinical medicine
Insulin resistance
Internal medicine
medicine
Animals
Insulin
Phosphorylation
Molecular Biology
Endoplasmic Reticulum Chaperone BiP
Heat-Shock Proteins
Benzofurans
Chemistry
Endoplasmic reticulum
Tunicamycin
Membrane Proteins
Cell Biology
medicine.disease
Endoplasmic Reticulum Stress
IRS1
Disease Models, Animal
030104 developmental biology
Endocrinology
Diabetes Mellitus, Type 2
Liver
030220 oncology & carcinogenesis
Unfolded protein response
Hepatic stellate cell
Hepatocytes
Insulin Receptor Substrate Proteins
Insulin Resistance
Transcription Factor CHOP
Signal Transduction
Subjects
Details
- ISSN :
- 10902104
- Volume :
- 526
- Issue :
- 3
- Database :
- OpenAIRE
- Journal :
- Biochemical and biophysical research communications
- Accession number :
- edsair.doi.dedup.....03ddfe6ee16cf7f8bd1aecc5ba907fda