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Investigating the dispersal of antibiotic resistance associated genes from manure application to soil and drainage waters in simulated agricultural farmland systems
- Source :
- PLoS ONE, PLoS ONE, Vol 14, Iss 9, p e0222470 (2019)
- Publication Year :
- 2019
-
Abstract
- Manure from animals that have been treated with antibiotics is often used to fertilize agricultural soils and its application has previously been shown to enrich for genes associated with antibiotic resistance in agroecosystems. To investigate the magnitude of this effect, we designed a column experiment simulating manure-treated agricultural soil that utilizes artificial subsurface drainage to determine the duration and extent which this type of manure fertilization impacts the set of genes associated with antibiotic resistance in drainage water. We classified ARGs in manure-treated drainage effluent water by its source of origin. Overall, we found that 61% and 7% of the total abundance of ARGs found in drainage water samples could be attributed to manure enrichment and manure addition, respectively. Among these ARGs, we identified 75 genes unique to manure that persisted in both soil and drainage water throughout a drainage season typical of the Upper Midwestern United States. While most of these genes gradually decreased in abundance over time, the IS6100-associated tet(33) gene accrued. These results demonstrate the influence of manure applications on the composition of the resistome observed in agricultural drainage water and highlight the importance of anthropogenic ARGs in the environment.
- Subjects :
- Molecular biology
Swine
Marine and Aquatic Sciences
DNA cloning
Midwestern United States
Soil
Antibiotics
Agricultural Soil Science
Limnology
Medicine and Health Sciences
Drainage
Soil Microbiology
Mammals
0303 health sciences
Multidisciplinary
Antimicrobials
Drugs
Eukaryota
Agriculture
Drug Resistance, Microbial
Genomics
Anti-Bacterial Agents
Agricultural soil science
Wastewater
Tetracyclines
Vertebrates
Medicine
Soil microbiology
Research Article
Farms
Science
Soil Science
Microbiology
03 medical and health sciences
Microbial Control
Genetics
Animals
Sequencing Techniques
Effluent
030304 developmental biology
Pharmacology
Shotgun Sequencing
030306 microbiology
Ecology and Environmental Sciences
Organisms
Biology and Life Sciences
Manure
Resistome
Research and analysis methods
Molecular biology techniques
Agronomy
Genes, Bacterial
Antibiotic Resistance
Soil water
Amniotes
Earth Sciences
Environmental science
Antimicrobial Resistance
Metagenomics
Cloning
Subjects
Details
- ISSN :
- 19326203
- Volume :
- 14
- Issue :
- 9
- Database :
- OpenAIRE
- Journal :
- PloS one
- Accession number :
- edsair.doi.dedup.....03cb5f325338335a736e68099e80abcb