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Systematic assessment of commercially available low-input miRNA library preparation kits
- Source :
- RNA Biology
- Publication Year :
- 2019
- Publisher :
- Informa UK Limited, 2019.
-
Abstract
- High-throughput sequencing is increasingly favoured to assay the presence and abundance of micro RNAs (miRNAs) in biological samples, even from low RNA amounts, and a number of commercial vendors now offer kits that allow miRNA sequencing from sub-nanogram (ng) inputs. However, although biases introduced during library preparation have been documented, the relative performance of current reagent kits has not been investigated in detail. Here, six commercial kits capable of handling
- Subjects :
- Library preparation
Sequencing bias
Total rna
Differentially expressed mirnas
Computational biology
Biology
DNA sequencing
03 medical and health sciences
0302 clinical medicine
Next generation sequencing
microRNA
Humans
Molecular Biology
Gene Library
030304 developmental biology
Low RNA input
0303 health sciences
VDP::Landbruks- og Fiskerifag: 900
Sequence Analysis, RNA
Mirna sequencing
Low input
High-Throughput Nucleotide Sequencing
Reproducibility of Results
RNA
MicroRNA
Cell Biology
UMI
MicroRNAs
NGS
030220 oncology & carcinogenesis
Genetic Engineering
MiRNA
Small RNA-seq
Research Paper
Subjects
Details
- ISSN :
- 15558584 and 15476286
- Volume :
- 17
- Database :
- OpenAIRE
- Journal :
- RNA Biology
- Accession number :
- edsair.doi.dedup.....03c3456cbd2400599807af6879b6614f