The contribution of genes required for anaerobic respiration to the virulence of Salmonella enterica serovar Gallinarum for chickens

Submitted by Guilherme Lemeszenski (guilherme@nead.unesp.br) on 2013-08-22T18:41:30Z No. of bitstreams: 1 S1517-83822009000400035.pdf: 137697 bytes, checksum: ac34dae451301dba8c4229402a3a02bb (MD5) Made available in DSpace on 2013-08-22T18:41:30Z (GMT). No. of bitstreams: 1 S1517-83822009000400035.pdf: 137697 bytes, checksum: ac34dae451301dba8c4229402a3a02bb (MD5) Previous issue date: 2009-12-01 Made available in DSpace on 2013-09-30T19:28:02Z (GMT). No. of bitstreams: 2 S1517-83822009000400035.pdf: 137697 bytes, checksum: ac34dae451301dba8c4229402a3a02bb (MD5) S1517-83822009000400035.pdf.txt: 30693 bytes, checksum: 16737efc25c24167d000935e52514418 (MD5) Previous issue date: 2009-12-01 Submitted by Vitor Silverio Rodrigues (vitorsrodrigues@reitoria.unesp.br) on 2014-05-20T13:15:54Z No. of bitstreams: 2 S1517-83822009000400035.pdf: 137697 bytes, checksum: ac34dae451301dba8c4229402a3a02bb (MD5) S1517-83822009000400035.pdf.txt: 30693 bytes, checksum: 16737efc25c24167d000935e52514418 (MD5) Made available in DSpace on 2014-05-20T13:15:54Z (GMT). No. of bitstreams: 2 S1517-83822009000400035.pdf: 137697 bytes, checksum: ac34dae451301dba8c4229402a3a02bb (MD5) S1517-83822009000400035.pdf.txt: 30693 bytes, checksum: 16737efc25c24167d000935e52514418 (MD5) Previous issue date: 2009-12-01 Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Salmonella enterica serovar Gallinarum (SG) is an intracellular pathogen of chickens. To survive, to invade and to multiply in the intestinal tract and intracellularly it depends on its ability to produce energy in anaerobic conditions. The fumarate reductase (frdABCD), dimethyl sulfoxide (DMSO)-trimethylamine N-oxide (TMAO) reductase (dmsABC), and nitrate reductase (narGHIJ) operons in Salmonella Typhimurium (STM) encode enzymes involved in anaerobic respiration to the electron acceptors fumarate, DMSO, TMAO, and nitrate, respectively. They are regulated in response to nitrate and oxygen availability and changes in cell growth rate. In this study mortality rates of chickens challenged with mutants of Salmonella Gallinarum, which were defective in utilising anaerobic electron acceptors, were assessed in comparison to group of bird challenged with wild strain. The greatest degree of attenuation was observed with mutations affecting nitrate reductase (napA, narG) with additional attenuations induced by a mutation affecting fumarate reductase (frdA) and a double mutant (dmsA torC) affecting DMSO and TMAO reductase. Universidade Estadual Paulista Faculdade de Ciências Agrárias e Veterinárias University of Nottingham School of Veterinary Medicine and Science Universidade Estadual Paulista Faculdade de Ciências Agrárias e Veterinárias