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SOX7 blocks vasculogenic mimicry in oral squamous cell carcinoma
- Source :
- Journal of Oral Pathology & Medicine. 50:766-775
- Publication Year :
- 2021
- Publisher :
- Wiley, 2021.
-
Abstract
- Background: Vasculogenic mimicry (VM) is the formation of an alternative circulatory system by aggressive tumor cells. The characteristics of VM and its underlying mechanism in oral squamous cell carcinoma (OSCC) remain unclear. This study aims to determine the relationship between VM channels in OSCC tissues and clinical outcomes and to investigate the biological role of SOX7 in VM in OSCC cells.Methods: CD31/PAS double staining was performed to evaluate VM status in OSCC tissue. The relationships between VM and clinicopathological variables, and VM and SOX7 levels were analyzed. The correlation between SOX7 levels and head and Neck cancer corhorts were investigated using in silico analysis.VM channel formation assay was performed to observe VM channels in the OSCC cell lines. To investigate the role of SOX7 in VM channel formation, SOX7 was transiently over-expressed in SCC-9 cells. VM-modulating genes were identified by Western blotting.Results: We confirmed the presence of VM channels in OSCC tissue and several cell lines and found a positive correlation between VM and lymph node metastasis and patient survival in OSCC (P = 0.003). In silico analysis from The Cancer Genome Atlas (TCGA) and Gene Expression Omnibus (GEO) database showed that down-regulation of SOX7 expression was significantly correlated with head and neck squamous cell carcinoma (HNSCC) patient cohorts (P = 0.0187) and lymph node metastasis (P = 0.0017). We also found that the presence of VM channels in OSCC tissue was inversely associated with SOX7 expression (P = 0.020). We observed that overexpression of SOX7 impaired VM channel formation by reducing the expression of VE-cadherin, thereby inhibiting cell migration and invasion.Conclusion: These results suggest that SOX7 plays an important role in the regulation of VM channel formation and may inhibit OSCC metastasis.
- Subjects :
- CD31
Cancer Research
Periodic acid–Schiff stain
Biology
Pathology and Forensic Medicine
Metastasis
03 medical and health sciences
0302 clinical medicine
Cell Line, Tumor
SOXF Transcription Factors
medicine
Humans
Vasculogenic mimicry
Neovascularization, Pathologic
Squamous Cell Carcinoma of Head and Neck
Cancer
Cell migration
030206 dentistry
medicine.disease
In vitro
Blot
stomatognathic diseases
Otorhinolaryngology
Head and Neck Neoplasms
030220 oncology & carcinogenesis
Carcinoma, Squamous Cell
Cancer research
Periodontics
Mouth Neoplasms
Oral Surgery
Subjects
Details
- ISSN :
- 16000714 and 09042512
- Volume :
- 50
- Database :
- OpenAIRE
- Journal :
- Journal of Oral Pathology & Medicine
- Accession number :
- edsair.doi.dedup.....03b9186ac61d795008e50e8adc30c90c