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Carbon metabolism and transcriptome in developmental paths differentiation of a homokaryotic Coprinopsis cinerea strain
- Source :
- Fungal Genetics and Biology. 143:103432
- Publication Year :
- 2020
- Publisher :
- Elsevier BV, 2020.
-
Abstract
- The balance and interplay between sexual and asexual reproduction is one of the most intriguing mysteries in the study of fungi. The choice of developmental strategy reflects the ability of fungi to adapt to the changing environment. However, the evolution of developmental paths and the metabolic regulation during differentiation and morphogenesis are poorly understood. Here, an analysis was performed of carbohydrate metabolism and gene expression regulation during the early differentiation process from the vegetative mycelium, to the differentiated structures, fruiting body, oidia and sclerotia, of a homokaryotic fruiting Coprinopsis cinerea strain A43mutB43mut pab1-1 #326. Changes during morphogenesis and the evolution of developmental strategies were followed. Conversion between glucose and glycogen and between glucose and beta-glucan were the main carbon flows in the differentiation processes. Genes related to carbohydrate transport and metabolism were significantly differentially expressed among paths. Sclerotia displayed a set of specifically up-regulated genes that were enriched in the carbon metabolism and energy production and conversion processes. Evolutionary transcriptomic analysis of four developmental paths showed that all transcriptomes were under the purifying selection, and the more stressful the environment, the younger the transcriptome age. Oidiation has the lowest value of transcriptome age index (TAI) and transcriptome divergence index (TDI), while the fruiting process has the highest of both indexes. These findings provide new insights into the regulations of carbon metabolism and gene expressions during the early stages of fungal developmental paths differentiation, and improve our understanding of the evolutionary process of life history and reproductive strategy in fungi.
- Subjects :
- Carbohydrate transport
Morphogenesis
Asexual reproduction
Biology
Microbiology
Fungal Proteins
Transcriptome
03 medical and health sciences
Gene Expression Regulation, Fungal
Genetics
Fruiting Bodies, Fungal
Mycelium
030304 developmental biology
Regulation of gene expression
0303 health sciences
030306 microbiology
Gene Expression Profiling
Cell Differentiation
biology.organism_classification
Carbon
Homokaryotic
Coprinopsis cinerea
Agaricales
Subjects
Details
- ISSN :
- 10871845
- Volume :
- 143
- Database :
- OpenAIRE
- Journal :
- Fungal Genetics and Biology
- Accession number :
- edsair.doi.dedup.....03b645a8e5a56b6f3fa67ebe14c03dab