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A Humoral Immune Response Alters the Distribution of Enzyme Replacement Therapy in Murine Mucopolysaccharidosis Type I

Authors :
Moin Vera
Terence M. Doherty
Shih hsin Kan
Kristen N. Vondrak
Michelina Iacovino
Martin T. Egeland
Steven Q. Le
Patricia I. Dickson
Jonathan D. Cooper
Mark S. Sands
Valentina Sanghez
Don Clarke
Source :
Molecular Therapy: Methods & Clinical Development, Vol 8, Iss C, Pp 42-51 (2018), Molecular Therapy. Methods & Clinical Development
Publication Year :
2018
Publisher :
Elsevier, 2018.

Abstract

Antibodies against recombinant proteins can significantly reduce their effectiveness in unanticipated ways. We evaluated the humoral response of mice with the lysosomal storage disease mucopolysaccharidosis type I treated with weekly intravenous recombinant human alpha-l-iduronidase (rhIDU). Unlike patients, the majority of whom develop antibodies to recombinant human alpha-l-iduronidase, only approximately half of the treated mice developed antibodies against recombinant human alpha-l-iduronidase and levels were low. Serum from antibody-positive mice inhibited uptake of recombinant human alpha-l-iduronidase into human fibroblasts by partial inhibition compared to control serum. Tissue and cellular distributions of rhIDU were altered in antibody-positive mice compared to either antibody-negative or naive mice, with significantly less recombinant human alpha-l-iduronidase activity in the heart and kidney in antibody-positive mice. In the liver, recombinant human alpha-l-iduronidase was preferentially found in sinusoidal cells rather than in hepatocytes in antibody-positive mice. Antibodies against recombinant human alpha-l-iduronidase enhanced uptake of recombinant human alpha-l-iduronidase into macrophages obtained from MPS I mice. Collectively, these results imply that a humoral immune response against a therapeutic protein can shift its distribution preferentially into macrophage-lineage cells, causing decreased availability of the protein to the cells that are its therapeutic targets.

Details

Language :
English
ISSN :
23290501
Volume :
8
Database :
OpenAIRE
Journal :
Molecular Therapy: Methods & Clinical Development
Accession number :
edsair.doi.dedup.....03b3e4be0fe91d6ae9802b52e6703012