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Deletion of Gb3 Synthase in Mice Resulted in the Attenuation of Bone Formation via Decrease in Osteoblasts
- Source :
- International Journal of Molecular Sciences, Vol 20, Iss 18, p 4619 (2019), International Journal of Molecular Sciences, Volume 20, Issue 18
- Publication Year :
- 2019
- Publisher :
- MDPI AG, 2019.
-
Abstract
- Glycosphingolipids are known to play a role in developing and maintaining the integrity of various organs and tissues. Among glycosphingolipids, there are several reports on the involvement of gangliosides in bone metabolism. However, there have been no reports on the presence or absence of expression of globo-series glycosphingolipids in osteoblasts and osteoclasts, and the involvement of their glycosphingolipids in bone metabolism. In the present study, we investigated the presence or absence of globo-series glycosphingolipids such as Gb3 (globotriaosylceramide), Gb4 (globoside), and Gb5 (galactosyl globoside) in osteoblasts and osteoclasts, and the effects of genetic deletion of Gb3 synthase, which initiates the synthesis of globo-series glycosphingolipids on bone metabolism. Among Gb3, Gb4, and Gb5, only Gb4 was expressed in osteoblasts. However, these glycosphingolipids were not expressed in pre-osteoclasts and osteoclasts. Three-dimensional micro-computed tomography (3D-&mu<br />CT) analysis revealed that femoral cancellous bone mass in Gb3 synthase-knockout (Gb3S KO) mice was lower than that in wild type (WT) mice. Calcein double labeling also revealed that bone formation in Gb3S KO mice was significantly lower than that in WT mice. Consistent with these results, the deficiency of Gb3 synthase in mice decreased the number of osteoblasts on the bone surface, and suppressed mRNA levels of osteogenic differentiation markers. On the other hand, osteoclast numbers on the bone surface and mRNA levels of osteoclast differentiation markers in Gb3S KO mice did not differ from WT mice. This study demonstrated that deletion of Gb3 synthase in mice decreases bone mass via attenuation of bone formation.
- Subjects :
- Male
musculoskeletal diseases
Globotriaosylceramide
Osteoclasts
digestive system
Article
Catalysis
Cell Line
Bone remodeling
Inorganic Chemistry
lcsh:Chemistry
Mice
chemistry.chemical_compound
globoside (Gb4)
Osteogenesis
Osteoclast
medicine
Animals
Bone formation
Physical and Theoretical Chemistry
Molecular Biology
lcsh:QH301-705.5
Cells, Cultured
Spectroscopy
Mice, Knockout
Globoside
glycosphingolipids
Chemistry
Organic Chemistry
Wild type
nutritional and metabolic diseases
osteoblasts
General Medicine
Galactosyltransferases
Molecular biology
Computer Science Applications
carbohydrates (lipids)
Calcein
RAW 264.7 Cells
medicine.anatomical_structure
lcsh:Biology (General)
lcsh:QD1-999
lipids (amino acids, peptides, and proteins)
bone metabolism
Cancellous bone
Gene Deletion
Subjects
Details
- Language :
- English
- ISSN :
- 14220067
- Volume :
- 20
- Issue :
- 18
- Database :
- OpenAIRE
- Journal :
- International Journal of Molecular Sciences
- Accession number :
- edsair.doi.dedup.....03b132c688b2d63fa9354d895c05c33a