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Establishment of a human squamous cell carcinoma cell line of the upper aero-digestive tract
- Source :
- Cancer letters. 118(1)
- Publication Year :
- 1997
-
Abstract
- A human squamous cell carcinoma (SCC) cell line has been established from the surgical specimen of an untreated, upper aero-digestive tract tumour, diagnosed as a squamous carcinoma, grade III, of the pyriform fossa. The tumour tissue was grown as a xenograft in an athymic nude mouse and was designated as NT-8. Histological examination of the surgical specimen and the nude mouse tumour showed that the two were identical. NT-8 was subsequently passed by subcutaneous injections into nude mice. After the 6th passage in nude mouse, the tumour was cultured in vitro where it grew as an epithelial cell line, with a typical cobblestone appearance. This cell line was designated as NT-8e. Both the primary tumour as well as xenograft and the cells in culture have retained several common morphological and biochemical characteristics. Immunological markers for epithelial cells including epithelial membrane antigen and cytokeratins were seen in all three, confirming the epithelial lineage. Characterization of the NT-8e cell line including growth parameters, anchorage-independent growth and tumorigenicity in nude mice, chromosome counts and DNA content by flow cytometry have been carried out.
- Subjects :
- Cancer Research
Pathology
medicine.medical_specialty
Transplantation, Heterologous
Cell Culture Techniques
Mice, Nude
Biology
Mice
Nude mouse
Proliferating Cell Nuclear Antigen
Carcinoma
medicine
Cell Adhesion
Tumor Cells, Cultured
Animals
Humans
Cell growth
Mucin-1
Chromosome Mapping
Pharyngeal Neoplasms
DNA, Neoplasm
Cell cycle
medicine.disease
biology.organism_classification
Squamous carcinoma
Transplantation
Kinetics
Oncology
Epidermoid carcinoma
Cell culture
Carcinoma, Squamous Cell
Keratins
Cell Division
Subjects
Details
- ISSN :
- 03043835
- Volume :
- 118
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Cancer letters
- Accession number :
- edsair.doi.dedup.....03a9e4c0d32af9c70d591cd7ce58b23a