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Palette of fluorinated voltage-sensitive hemicyanine dyes
- Source :
- Yan, P, Acker, C D, Zhou, W L, Lee, P, Bollensdorff, C, Negrean, A, Lotti, J, Sacconi, L, Antic, S D, Kohl, P, Mansvelder, H D, Pavone, F S & Loew, L M 2012, ' Palette of fluorinated voltage-sensitive hemicyanine dyes ', Proceedings of the National Academy of Sciences of the United States of America, vol. 109, no. 50, pp. 20443-20448 . https://doi.org/10.1073/pnas.1214850109, Proceedings of the National Academy of Sciences of the United States of America 109 (2012): 20443–20448. doi:10.1073/pnas.1214850109, info:cnr-pdr/source/autori:Yan Ping [ 1 ] ; Acker Corey D. [ 1 ] ; Zhou Wen-Liang [ 2 ] ; Lee Peter [ 3 ] ; Bollensdorff Christian [ 4 ] ; Negrean Adrian [ 5 ] ; Lotti Jacopo [ 6 ] ; Sacconi Leonardo [ 6,8 ] ; Antic Srdjan D. [ 2 ] ; Kohl Peter [ 4 ] ; Mansvelder Huibert D. [ 5 ] ; Pavone Francesco S. [ 6,7,8,9 ] ; Loew Leslie M. [ 1 ]/titolo:Palette of fluorinated voltage-sensitive hemicyanine dyes/doi:10.1073%2Fpnas.1214850109/rivista:Proceedings of the National Academy of Sciences of the United States of America/anno:2012/pagina_da:20443/pagina_a:20448/intervallo_pagine:20443–20448/volume:109, Proceedings of the National Academy of Sciences, Proceedings of the National Academy of Sciences of the United States of America, 109(50), 20443-20448. National Acad Sciences
- Publication Year :
- 2012
-
Abstract
- Optical recording of membrane potential permits spatially resolved measurement of electrical activity in subcellular regions of single cells, which would be inaccessible to electrodes, and imaging of spatiotemporal patterns of action potential propagation in excitable tissues, such as the brain or heart. However, the available voltage-sensitive dyes (VSDs) are not always spectrally compatible with newly available optical technologies for sensing or manipulating the physiological state of a system. Here, we describe a series of 19 fluorinated VSDs based on the hemicyanine class of chromophores. Strategic placement of the fluorine atoms on the chromophores can result in either blue or red shifts in the absorbance and emission spectra. The range of one-photon excitation wavelengths afforded by these new VSDs spans 440–670 nm; the two-photon excitation range is 900–1,340 nm. The emission of each VSD is shifted by at least 100 nm to the red of its one-photon excitation spectrum. The set of VSDs, thus, affords an extended toolkit for optical recording to match a broad range of experimental requirements. We show the sensitivity to voltage and the photostability of the new VSDs in a series of experimental preparations ranging in scale from single dendritic spines to whole heart. Among the advances shown in these applications are simultaneous recording of voltage and calcium in single dendritic spines and optical electrophysiology recordings using two-photon excitation above 1,100 nm.
- Subjects :
- Optical Phenomena
Dendritic Spines
CHARGE-SHIFT PROBES
ACTION-POTENTIALS
Guinea Pigs
Action Potentials
In Vitro Techniques
Biophysical Phenomena
Absorbance
Mice
Purkinje Cells
03 medical and health sciences
0302 clinical medicine
Optics
Microscopy
Animals
Calcium Signaling
Emission spectrum
Rats, Wistar
Coloring Agents
Fluorescent Dyes
030304 developmental biology
ELECTROCHROMIC MECHANISM
0303 health sciences
Multidisciplinary
Molecular Structure
business.industry
Chemistry
Brain
Heart
Fluorine
Carbocyanines
Biological Sciences
Chromophore
Fluorescence
Rats
Optical phenomena
Microscopy, Fluorescence, Multiphoton
Spectrophotometry
Optical recording
2-PHOTON EXCITATION
Female
POTENTIOMETRIC RESPONSES
business
030217 neurology & neurosurgery
Excitation
Subjects
Details
- Language :
- English
- ISSN :
- 00278424
- Database :
- OpenAIRE
- Journal :
- Yan, P, Acker, C D, Zhou, W L, Lee, P, Bollensdorff, C, Negrean, A, Lotti, J, Sacconi, L, Antic, S D, Kohl, P, Mansvelder, H D, Pavone, F S & Loew, L M 2012, ' Palette of fluorinated voltage-sensitive hemicyanine dyes ', Proceedings of the National Academy of Sciences of the United States of America, vol. 109, no. 50, pp. 20443-20448 . https://doi.org/10.1073/pnas.1214850109, Proceedings of the National Academy of Sciences of the United States of America 109 (2012): 20443–20448. doi:10.1073/pnas.1214850109, info:cnr-pdr/source/autori:Yan Ping [ 1 ] ; Acker Corey D. [ 1 ] ; Zhou Wen-Liang [ 2 ] ; Lee Peter [ 3 ] ; Bollensdorff Christian [ 4 ] ; Negrean Adrian [ 5 ] ; Lotti Jacopo [ 6 ] ; Sacconi Leonardo [ 6,8 ] ; Antic Srdjan D. [ 2 ] ; Kohl Peter [ 4 ] ; Mansvelder Huibert D. [ 5 ] ; Pavone Francesco S. [ 6,7,8,9 ] ; Loew Leslie M. [ 1 ]/titolo:Palette of fluorinated voltage-sensitive hemicyanine dyes/doi:10.1073%2Fpnas.1214850109/rivista:Proceedings of the National Academy of Sciences of the United States of America/anno:2012/pagina_da:20443/pagina_a:20448/intervallo_pagine:20443–20448/volume:109, Proceedings of the National Academy of Sciences, Proceedings of the National Academy of Sciences of the United States of America, 109(50), 20443-20448. National Acad Sciences
- Accession number :
- edsair.doi.dedup.....03a1d6378c2fc388f6f9a0c9714e6c90