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Quercetin regulates inflammation, oxidative stress, apoptosis, and mitochondrial structure and function in H9C2 cells by promoting PVT1 expression
- Source :
- Acta histochemica. 123(8)
- Publication Year :
- 2021
-
Abstract
- OBJECTIVE To investigate the effect and potential mechanism of quercetin on inflammation, oxidative stress, apoptosis, and mitochondrial structure and function in H9C2 cells. MATERIALS AND METHODS H9C2 cells were obtained from the Shanghai Institutes for Biological Sciences, Chinese Academy of Science, and randomly divided into six groups: control, model, PVT1 overexpression (OV), quercetin, OV + quercetin, and NAC groups. The CCK-8 assay was performed to examine cell proliferation. Flow cytometry was used to examine cell apoptosis, cell membrane potential, and ROS levels. The expression of endothelial nitric oxide synthase (eNOS), malondialdehyde (MDA), and superoxide dismutase (SOD) was measured by ELISA and a Biochemical kit. Western blotting was used to determine the levels of p-DRP1 (s637), MFN2, NF-kB, p-NF-kB, IkB, and p-IkB. IL-6, IL-10, TNF-α, and IL-1β mRNA expression was examined by RT-PCR. Electron microscopy was used to observe the structure of mitochondria in H9C2 cells. RESULTS MDA, p-NF-κB, p-IKB, IL-6, IL-1β, and TNF-α expression levels, and the cell apoptosis rate were significantly higher in the model group than in the control group (P
- Subjects :
- Histology
MFN2
Apoptosis
Mitochondrion
medicine.disease_cause
Mitochondria, Heart
Cell Line
Superoxide dismutase
medicine
Humans
Inflammation
biology
Chemistry
Cell growth
Cell Biology
General Medicine
Molecular biology
Oxidative Stress
mitochondrial fusion
Gene Expression Regulation
biology.protein
Mitochondrial fission
Quercetin
RNA, Long Noncoding
Oxidative stress
Subjects
Details
- ISSN :
- 16180372
- Volume :
- 123
- Issue :
- 8
- Database :
- OpenAIRE
- Journal :
- Acta histochemica
- Accession number :
- edsair.doi.dedup.....03a0748ed80f1ec822582a58e63aa4a1