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The impact of dietary fermentable carbohydrates on a postinflammatory model of irritable bowel syndrome
- Source :
- Neurogastroenterology and motility : the official journal of the European Gastrointestinal Motility SocietyREFERENCES. 31(10)
- Publication Year :
- 2019
-
Abstract
- BACKGROUND A low fermentable carbohydrate (FODMAP) diet is used in quiescent inflammatory bowel disease when irritable bowel syndrome-like symptoms occur. There is concern that the diet could exacerbate inflammation by modifying microbiota and short-chain fatty acid (SCFA) production. We examined the effect of altering dietary FODMAP content on inflammation in preclinical inflammatory models. METHODS C57BL/6 mice were given 3% dextran sodium sulfate (DSS) in drinking water for 5 days and recovered for 3 weeks (postinflammatory, n = 12), or 5 days (positive-control, n = 12). Following recovery, DSS-treated or control mice (negative-control, n = 12) were randomized to 2-week low- (0.51 g/100 g total FODMAP) or high-FODMAP (4.10 g) diets. Diets mimicked human consumption containing fructose, sorbitol, galacto-oligosaccharide, and fructan. Colons were assessed for myeloperoxidase (MPO) activity and histological damage. Supernatants were generated for perforated patch-clamp recordings and cytokine measurement. Cecum contents were analyzed for microbiota, SCFA, and branched-chain fatty acids (BCFA). Data were analyzed by two-way ANOVA with Bonferroni. KEY RESULTS Inflammatory markers were higher in the positive-control compared with negative-control and postinflammatory groups, but no differences occurred between the two diets within each treatment (MPO P > .99, histological scores P > .99, cytokines P > .05), or the perforated patch-clamp recordings (P > .05). Microbiota clustered mainly based on DSS exposure. No difference in SCFA content occurred. Higher total BCFA occurred with the low-FODMAP diet in positive-control (P
- Subjects :
- 0301 basic medicine
Nociception
Patch-Clamp Techniques
Physiology
Oligosaccharides
Disaccharides
Inflammatory bowel disease
Gastroenterology
Irritable Bowel Syndrome
Cecum
Mice
0302 clinical medicine
Isobutyrates
RNA, Ribosomal, 16S
Irritable bowel syndrome
chemistry.chemical_classification
biology
Dextran Sulfate
Fatty Acids
Monosaccharides
Colitis
medicine.anatomical_structure
Myeloperoxidase
Cytokines
030211 gastroenterology & hepatology
medicine.symptom
FODMAP
medicine.medical_specialty
Inflammation
03 medical and health sciences
Hemiterpenes
Internal medicine
medicine
Dietary Carbohydrates
Animals
Pentanoic Acids
Peroxidase
Endocrine and Autonomic Systems
business.industry
Fatty acid
Carbohydrate
medicine.disease
Fatty Acids, Volatile
Inflammatory Bowel Diseases
Gastrointestinal Microbiome
Disease Models, Animal
030104 developmental biology
chemistry
Fermentation
biology.protein
business
Subjects
Details
- ISSN :
- 13652982
- Volume :
- 31
- Issue :
- 10
- Database :
- OpenAIRE
- Journal :
- Neurogastroenterology and motility : the official journal of the European Gastrointestinal Motility SocietyREFERENCES
- Accession number :
- edsair.doi.dedup.....039b182d2ddb92b178e4649cec58237d