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Simultaneous liquid chromatographic determination of residual synthetic antibacterials in cultured fish

Simultaneous liquid chromatographic determination of residual synthetic antibacterials in cultured fish

Authors :
Masakazu Horie
Youji Hoshino
Yoshinori Kikuchi
Kouichi Saitoh
Takashi Kawachi
Norihide Nose
Hiroyuki Nakazawa
Source :
Journal - Association of Official Analytical Chemists. 70(4)
Publication Year :
1987

Abstract

A liquid chromatographic method is described to determine simultaneously the following 11 synthetic antibacterial agents used in a fishery: nitrofuran derivatives furazolidone, nifurpirinol, difurazone, and furamizole; sulfa drugs sulfamerazine, sulfisozole, sulfamonomethoxine, and sulfadimethoxine; and, oxolinic, nalidixic, and piromidic acids. A Nucleosil C18 column was used with tetrahydrofuran- acetonitrile-phosphoric acid-water (29 + 1 + 0.06 + 69.94) as the mobile phase. Pretreatment of the fish meat sample with acetone extraction and alumina column cleanup gave good separation of the LC peaks without interference from any other components. Recovery of the antibacterial agents was ca 80%. The lower limit of detection of the drugs was 1-2 ng for 10 μL injection.

Details

ISSN :
00045756
Volume :
70
Issue :
4
Database :
OpenAIRE
Journal :
Journal - Association of Official Analytical Chemists
Accession number :
edsair.doi.dedup.....0381baa7b12796069a6a283e05cf37ae