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Simultaneous liquid chromatographic determination of residual synthetic antibacterials in cultured fish
Simultaneous liquid chromatographic determination of residual synthetic antibacterials in cultured fish
- Source :
- Journal - Association of Official Analytical Chemists. 70(4)
- Publication Year :
- 1987
-
Abstract
- A liquid chromatographic method is described to determine simultaneously the following 11 synthetic antibacterial agents used in a fishery: nitrofuran derivatives furazolidone, nifurpirinol, difurazone, and furamizole; sulfa drugs sulfamerazine, sulfisozole, sulfamonomethoxine, and sulfadimethoxine; and, oxolinic, nalidixic, and piromidic acids. A Nucleosil C18 column was used with tetrahydrofuran- acetonitrile-phosphoric acid-water (29 + 1 + 0.06 + 69.94) as the mobile phase. Pretreatment of the fish meat sample with acetone extraction and alumina column cleanup gave good separation of the LC peaks without interference from any other components. Recovery of the antibacterial agents was ca 80%. The lower limit of detection of the drugs was 1-2 ng for 10 μL injection.
- Subjects :
- Detection limit
Sulfamerazine
Chromatography
Furazolidone
Extraction (chemistry)
Fishes
Sulfadimethoxine
General Chemistry
chemistry.chemical_compound
chemistry
Anti-Infective Agents
medicine
Acetone
Sulfamonomethoxine
Animals
Indicators and Reagents
Spectrophotometry, Ultraviolet
Antibacterial agent
medicine.drug
Chromatography, Liquid
Subjects
Details
- ISSN :
- 00045756
- Volume :
- 70
- Issue :
- 4
- Database :
- OpenAIRE
- Journal :
- Journal - Association of Official Analytical Chemists
- Accession number :
- edsair.doi.dedup.....0381baa7b12796069a6a283e05cf37ae