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Engineering At-Home Dilution and Filtration Methods to Enable Paper-Based Colorimetric Biosensing in Human Blood with Cell-Free Protein Synthesis
- Source :
- Biosensors; Volume 13; Issue 1; Pages: 104
- Publication Year :
- 2023
- Publisher :
- MDPI AG, 2023.
-
Abstract
- Diagnostic blood tests can guide the administration of healthcare to save and improve lives. Most clinical biosensing blood tests require a trained technician and specialized equipment to process samples and interpret results, which greatly limits test accessibility. Colorimetric paper-based diagnostics have an equipment-free readout, but raw blood obscures a colorimetric response which has motivated diverse efforts to develop blood sample processing techniques. This work uses inexpensive readily-available materials to engineer user-friendly dilution and filtration methods for blood sample collection and processing to enable a proof-of-concept colorimetric biosensor that is responsive to glutamine in 50 µL blood drop samples in less than 30 min. Paper-based user-friendly blood sample collection and processing combined with CFPS biosensing technology represents important progress towards the development of at-home biosensors that could be broadly applicable to personalized healthcare.
Details
- ISSN :
- 20796374
- Volume :
- 13
- Database :
- OpenAIRE
- Journal :
- Biosensors
- Accession number :
- edsair.doi.dedup.....037e756eb65e97b7f0d30a8cbe74e995