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Expression of the pituitary transcription factor GHF-1/PIT-1 in cell types of the adult porcine adenohypophysis
- Source :
- The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society. 44(6)
- Publication Year :
- 1996
-
Abstract
- We describe the expression of the transcription factor GHF-1/PIT-1 in adult porcine adenohypophysis by a nonradioactive in situ hybridization (ISH) method using a digoxigenin-labeled cDNA probe corresponding to the entire coding region of rat GHF-1. GHF-1 transcripts were found in 71.7% of adenohypophyseal cells. We also report the simultaneous detection of GHF-1 mRNA and pituitary hormones by combined ISH and immunocytochemistry (IC) in dispersed adenohypophyseal cells, detected with an alkaline phosphatase-NBT/BCIP technique and with an immunoperoxidase-3-amino-9-ethylcarbazole (AEC) method, respectively. The combination of the two techniques neither abolished nor diminished their sensitivity or specificity. GHF-1 is expressed in all five of the cell types in the adult porcine adenohypophysis, showing that this method is suitable for simultaneous detection of transcripts and proteins at the single-cell level.
- Subjects :
- Messenger RNA
Pituitary gland
Histology
Swine
Immunocytochemistry
Gene Expression
In situ hybridization
Biology
Molecular biology
Cell Line
DNA-Binding Proteins
Immunoenzyme Techniques
medicine.anatomical_structure
Cell culture
Pituitary Gland, Anterior
Complementary DNA
Gene expression
medicine
Animals
Humans
Anatomy
Transcription Factor Pit-1
Transcription factor
In Situ Hybridization
Transcription Factors
Subjects
Details
- ISSN :
- 00221554
- Volume :
- 44
- Issue :
- 6
- Database :
- OpenAIRE
- Journal :
- The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society
- Accession number :
- edsair.doi.dedup.....03798f9ee76075c47c8328c7829ef609