Back to Search
Start Over
Discovery and Qualification of Serum Protein Biomarker Candidates for Cholangiocarcinoma Diagnosis
- Source :
- Journal of Proteome Research. 18:3305-3316
- Publication Year :
- 2019
- Publisher :
- American Chemical Society (ACS), 2019.
-
Abstract
- Cholangiocarcinoma (CCA) is a major health problem in northeastern Thailand. The majority of CCA cases are clinically silent and difficult to detect at an early stage. Although abdominal ultrasonography (US) can detect premalignant periductal fibrosis (PDF), this method is not suitable for screening populations in remote areas. With the goal of developing a blood test for detecting CCA in the at-risk population, we carried out serum protein biomarker discovery and qualification. Label-free shotgun proteomics was performed on depleted serum samples from 30 participants (n = 10 for US-normal, US-PDF, and CCA groups). Of 40 protein candidates selected using multiple reaction monitoring on 90 additional serum samples (n = 30 per group), 11 discriminatory proteins were obtained using supervised multivariate statistical analysis. We further evaluated 3 candidates using ELISA and immunohistochemistry (IHC). S100A9, thioredoxin (TRX), and cadherin-related family member 2 (CDHR2) were significantly different between CCA and normal, and CCA and PDF groups when measured in an additional 247 serum samples (P < 0.0001). By IHC, TRX and CDHR2 were detected in the cytoplasm and nucleus of CCA and inflammatory cells. S100A9 was detected in the infiltrating tumor stroma immune cells. Proteomics discovery and qualification in depleted sera revealed promising biomarker candidates for CCA diagnosis.
- Subjects :
- Male
Proteomics
0301 basic medicine
Pathology
medicine.medical_specialty
Population
Biochemistry
S100A9
Cholangiocarcinoma
03 medical and health sciences
Risk Factors
parasitic diseases
Biomarkers, Tumor
Humans
Medicine
Blood test
Biomarker discovery
education
Shotgun proteomics
Aged
Ultrasonography
education.field_of_study
030102 biochemistry & molecular biology
medicine.diagnostic_test
business.industry
fungi
General Chemistry
Middle Aged
Neoplasm Proteins
Bile Ducts, Intrahepatic
030104 developmental biology
Immunohistochemistry
Biomarker (medicine)
Female
business
Precancerous Conditions
Subjects
Details
- ISSN :
- 15353907 and 15353893
- Volume :
- 18
- Database :
- OpenAIRE
- Journal :
- Journal of Proteome Research
- Accession number :
- edsair.doi.dedup.....035b328dd1d69865f000c12300f73db8