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Ultrasensitive thrombin detection based on direct electrochemistry of highly loaded hemoglobin spheres-encapsulated platinum nanoparticles as labels and electrocatalysts
- Source :
- Biosensors and Bioelectronics. 50:50-56
- Publication Year :
- 2013
- Publisher :
- Elsevier BV, 2013.
-
Abstract
- For the first time, a sandwich-type electrochemical method was proposed for ultrasensitive thrombin (TB) detection based on direct electrochemistry of highly loaded hemoglobin spheres-encapsulated platinum nanoparticles (PtNPs@Hb) as labels and electrocatalysts. The prepared PtNPs@Hb not only exhibited good biocompatibility, excellent electrocatalytic activity, but also presented redox activity of Hb. Thus, it was employed for the fabrication of aptasensor without any extraneous redox mediators, leading to a simple preparation process for the aptasensor. The high loading of Hb spheres as redox mediators could enhance the electrochemical signal. Importantly, the synergetic electrocatalytic behavior of Hb and PtNPs toward H2O2 reduction greatly amplified the electrochemical signal, resulting in the high sensitivity of aptasensor. Consequently, under optimal conditions, the designed aptasensor exhibited a lower detection limit of 0.05 pM and wide dynamic linear range from 0.15 pM to 40 nM for TB detection. Additionally, the proposed mediator-free and signal-amplified electrochemical aptasensor showed great potential in portable and cost-effective TB sensing devices.
- Subjects :
- Models, Molecular
Materials science
Biocompatibility
Biomedical Engineering
Biophysics
Nanotechnology
Biosensing Techniques
Electrochemistry
Platinum nanoparticles
Redox
Catalysis
Hemoglobins
Thrombin
Limit of Detection
medicine
Humans
Platinum
Detection limit
Electrochemical Techniques
General Medicine
Aptamers, Nucleotide
Linear range
Nanoparticles
Hemoglobin
Biotechnology
medicine.drug
Subjects
Details
- ISSN :
- 09565663
- Volume :
- 50
- Database :
- OpenAIRE
- Journal :
- Biosensors and Bioelectronics
- Accession number :
- edsair.doi.dedup.....034dfad001ad2d3fd09c654727ebb63b