Measurement of pH Values in Human Tissues by Two-Photon Microscopy

Authors :: Eun Sun Kim
Hoon Jai Chun
Bong Rae Cho
Hee Jung Park
Tae Hee Lee
Chang Su Lim
Ji Hee Han
Source :: Angewandte Chemie International Edition. 51:2673-2676
Publication Year :: 2012
Publisher :: Wiley, 2012.
Abstract: pH values go live! A ratiometric two-photon (TP) probe (NP1, see scheme) that has a significant TP action cross-section, high photostability, negligible toxicity, and can estimate pH values in live cells and human tissues by two-photon microscopy is described. NP1 can detect the difference in pH between live cells from the gastroesophageal junction (GEJ) and the lower esophageal sphincter of patients with and without esophagitis.