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Flow cytometry as a diagnostic tool for identifying total hip arthroplasty loosening and differentiating between septic and aseptic cases
- Source :
- European Journal of Orthopaedic Surgery & Traumatology. 25:1153-1159
- Publication Year :
- 2015
- Publisher :
- Springer Science and Business Media LLC, 2015.
-
Abstract
- Purpose Implant loosening represents one of the major factors of total hip arthroplasty (THA) failure. The purpose of this study was to identify specific markers indicative of septic and aseptic loosening in patients that underwent THA. Methods Flow cytometry was performed in blood samples of 20 patients with loosening (10 septic/10 aseptic). Additional ten healthy individuals served as a control group. The expression of surface receptors and cytoplasmic molecules in patients that underwent THA was quantified. CD62L, CD18, CD11a, CD11b and CD11c expressions were evaluated and correlated with the presence of loosening. Also, a comparison between septic and aseptic THA loosening characteristics was performed. Results The mean fluorescence intensity (MFI) for CD18 was significantly decreased on all leukocytes subsets in both septic and aseptic loosening compared to control group (p\0.005 in all occasions). Patients with aseptic loosening showed increased MFI for CD11b in granulocytes and for CD11c in monocytes and granulocytes compared to the control and aseptic group (p = 0.02 and p = 0.005, respectively). In patients with septic loosening, an increase in MFI for CD11c was observed in monocytes only compared to control group (p = 0.03). The comparison between aseptic and septic loosening showed significantly lower CD18 MFI value in granulocytes for aseptic loosening (p = 0.008). Conclusions CD11 and CD18 MFI values appear to be indicative of loosening in THAs. Flow cytometry markers can be used to identify THA loosening, as well as to differentiate between septic and aseptic cases.
- Subjects :
- Adult
Male
musculoskeletal diseases
medicine.medical_specialty
Arthroplasty, Replacement, Hip
Aseptic loosening
Flow cytometry
Sepsis
medicine
Humans
Orthopedics and Sports Medicine
In patient
L-Selectin
Aged
medicine.diagnostic_test
CD11 Antigens
business.industry
Mean fluorescence intensity
Middle Aged
Flow Cytometry
musculoskeletal system
equipment and supplies
medicine.disease
Prosthesis Failure
Surgery
surgical procedures, operative
CD18 Antigens
Case-Control Studies
Female
Hip Prosthesis
Implant
Aseptic processing
business
Total hip arthroplasty
Subjects
Details
- ISSN :
- 14321068 and 16338065
- Volume :
- 25
- Database :
- OpenAIRE
- Journal :
- European Journal of Orthopaedic Surgery & Traumatology
- Accession number :
- edsair.doi.dedup.....03418f6143a2cd8f3df987e186172268